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Cow's milk-based functional foods that can prevent gastroenteritis

可预防胃肠炎的牛奶功能食品

基本信息

批准号：
11556048
负责人：
KANAMARU Yoshihiro
金额：
$ 8.32万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

A research work was carried out ( i ) to examine the efficacy of cow's milk proteins to prevent infections both in vitro and in experimental animal infection models in vivo, ( ii ) to obtain proper prescriptions, and ( iii ) finally to produce a cow's milk-based functional food that can prevent gastroenteritis. Human rotavirus ( HRV ) and Helicobacterpylori ( Hp ) were the target causative agents. The following results were obtained.I. Prevention of HRV infection: In vitro neutralization test revealed that the high-Mr whey protein concentrate ( HWPC_<300> ) containing milk mucin complex ( MMC ) obtained by ultra filtration/diafiltration or its void volume fraction ( Fl ) from Sephacryl S-500 gel filtration is highly potent in inhibiting HRV infection. Moreover, a fraction containing some low-Mr proteins dissociated from MMC with detergent was found to be as potent as the specific antibodies from a colostrum of the cow hyperimmunized with HRV ( Rotamilk IgG ). In the experimental animal infection model, it was clearly demonstrated that orally administered HWPCjoo, as well as Rotamilk IgG, prevented diarrhea in suckling mice caused by HRV. A minimum dose of HWPC_(300) in infants may be 10 g a day while 500 mg of Rotamilk IgG may be enough forprevention from diarrhea.II. Prevention of HP infection: The ability to inhibit adherence of Hp urease to gastric mucin in vitro was tested with Fl and its fractions from gel filtration in the presence of detergent. Comparing to the inhibiting activity of dextran sulfate ( DS ), it was demonstrated that IC_<50> of F1 was 〜100 fold lower and those of its fractions containing milk mucin were 〜1000 fold lower than that of DS. Since it was already reported that 2.5 % DS in feed could prevent Hp colonization in euthymic hairless mice, it can be concluded that Fl and its milk mucin containing fractions would be able to prevent infection of Hp in vivo.

开展了以下研究工作：(1)在体外和体内实验动物感染模型中检查牛奶蛋白预防感染的功效；(2)获得适当的处方；(3)最终生产出以牛奶为基础的预防肠胃炎的功能性食品。人类轮状病毒（HRV）和幽门螺杆菌（Hp）是目标病原体。得到了以下结果：1。预防HRV感染：体外中和试验表明，通过超滤/滤除或其空隙体积分数（Fl）从sepphacryl S-500凝胶过滤得到的含有乳粘蛋白复合物（MMC）的高mr乳清蛋白浓缩物（HWPC_<300>）对HRV感染具有很强的抑制作用。此外，发现含有一些用洗涤剂从MMC解离的低mr蛋白的部分与从HRV过度免疫的牛初乳中提取的特异性抗体（rotammilk IgG）一样有效。在实验动物感染模型中，清楚地表明，口服HWPCjoo和rotammilk IgG可以预防HRV引起的哺乳小鼠腹泻。婴儿最低剂量的HWPC_（300）可能是每天10克，而500毫克的rotammilk IgG可能足以预防腹泻。预防HP感染：体外抑制HP脲酶粘附胃粘蛋白的能力用Fl及其在洗涤剂存在下凝胶过滤的组分进行了测试。与硫酸葡聚糖（DS）的抑制活性相比，F1的IC_<50>比DS低100倍，其含乳粘蛋白组分的IC_<50>比DS低1000倍。已有报道称，饲料中添加2.5% DS可抑制Hp在无毛小鼠体内的定殖，可见Fl及其乳粘蛋白组分可抑制Hp在体内的感染。