LC-ICP-MS/MS System

本申请的主题是采购LC-ICP-MS/MS系统，用于分析人体和环境样品中超痕量范围的微量元素。申请人无法获得这一技术，现有仪器在灵敏度、特异性和通量方面不符合最先进元素分析的要求。LC-ICP-MS/MS系统能够检测低至亚ppt范围的元素及其种类，并在无干扰的情况下进行分析。因此，它们非常适合于执行申请中列出的项目，并且对于申请研究小组在微量元素研究方面的未来生存能力至关重要。除其他外，由于必须在不同基质中测定少量分析物，因此需要购买LC-ICP-MS/MS系统。上述研究领域之一是建立微量取样方法，对血液和尿液中的有毒金属进行生物监测。微量取样只需要非常少量的基质（< 100 µl），因此非常适合用于大量样品收集或储存有问题的研究，例如在发展中国家。因此，必须满足分析灵敏度方面的最高要求，以便即使在最低浓度下也能检测到痕量元素。此外，还将评价微量取样方法对形态分析的适用性。这就需要将ICP-MS/MS与色谱法结合起来。进一步的项目包括对来自研究的生物样品进行多元素测定，有时需要数千名测试人员。ICP-MS/MS系统适用于高通量分析，因为它们可以在一次测定中测量多个元素，因此每个样品所需的时间相对较低。如果需要，可以在选定的样品中确定元素的种类，以详细评价毒理学相关性。另一个研究领域是微量元素对某些疾病的影响。由于这部分是在细胞和小鼠模型中进行的研究，通常只有微量样品，因此也必须满足微量元素分析的最高要求。总之，通过购买LC-ICP-MS/MS系统，参与应用的工作组可以更有效地开展现有项目，并灵活地应对新的研究问题。此外，它们还准备满足今后几年的要求。此外，联合使用该系统为各工作组之间的合作开辟了新的机会。集成到LMU Klinikum的核心设施中，使所有感兴趣的研究小组都可以进行微量元素分析。

The subject of this application is the procurement of an LC-ICP-MS/MS system for the analysis of trace elements in human and environmental samples in the ultra-trace range. Access to this technology is not available to the applicants and the instrumentation available does not meet the requirements of state-of-the-art elemental analysis in terms of sensitivity, specificity and throughput. LC-ICP-MS/MS systems are able to detect elements and their species down to the sub-ppt range and analyse them without interference. Thus, they are ideally suited for the execution of the projects listed in the application and are essential for the future viability of the applying research groups with regard to trace element research.The necessity to acquire an LC-ICP-MS/MS system is, among other things, due to the small amounts of analytes that have to be determined in different matrices. One of the research fields described above involves the establishment of microsampling methods for the biomonitoring of toxic metals in blood and urine. Microsampling requires only very small amounts of matrix (< 100 µl) and is therefore ideally used for studies where the collection of large amounts of sample or its storage is a problem, e.g. in developing countries. Consequently, highest demands must be met regarding the sensitivity of the analysis so that trace elements can be detected even at the lowest concentrations. Furthermore, the suitability of microsampling methods for speciation analysis will be evaluated. This requires the coupling of ICP-MS/MS with chromatography.Further projects include multi-element determinations in biosamples from studies with sometimes thousands of test persons. ICP-MS/MS systems are suitable for high throughput analysis because they can measure several elements in one determination and the time required per sample is therefore relatively low. If required, the species of the elements can be determined in selected samples to evaluate the toxicological relevance in detail. Another field of research is the influence of trace elements in certain diseases. As this is partly investigated in cell and mouse models, in which often only micro samples are available, the highest demands on trace element analysis must be met here as well.In summary, by acquiring an LC-ICP-MS/MS system, the working groups involved in the application can work on current projects more effectively and efficiently and react flexibly to new research questions. In addition, they are prepared to meet future requirements for years to come. Furthermore, the joint use of the system opens up new opportunities for collaborations between the working groups. The integration into the core facility of the LMU Klinikum makes trace element analysis accessible to all interested research groups.