Method Development/Validation Multi-laboratory validation of method for Arsenic speciation in seafood using LC-ICP-MS (Discipline D: Special Projects Analytical Track 3)

项目摘要 这一特别项目的总体目标是对以下方面的可行性进行初步的探索性调查： 建立了一种基于简单提取方法的海产品中砷形态分析的新方法， 使用LC-ICP-MS进行分离和检测。拟议的项目将利用现有的专业知识， 沃兹沃斯中心的能力，该中心已经开发出一种简单的LC-ICP-MS方法， 其可以被多个实验室验证以在FERN网络上使用。具体目标将 包括探索通过将As（III）氧化为As（V）来结合As（III）和As（V）的可行性，以产生 单一无机As物种（iAs）。其他目标将包括决定有多少不同的物种应该 在所提出的方法中捕获，而识别校准标准仅存在于有限数量的As中 物种一个关键的目标将是产生一个合适的候选参考材料的基础上， 产品矩阵可以被均质化，并分配到多个实验室进行首次实验室间 study.其他目标将包括一个单一的实验室验证所提出的方法，和设计的 第一次实验室间循环，收集网络能力的基线数据

PROJECT SUMMARY The overall goal of this special project is to conduct a preliminary, exploratory investigation of the feasibility of developing a new method for arsenic (As) speciation in seafood based on a simple extraction procedure with separation and detection using LC-ICP-MS. The proposed project will leverage the current expertise and capabilities at the Wadsworth Center, where a simple LC-ICP-MS method has already been developed, and which could be validated for use across the FERN network by multiple laboratories. Specific objectives will include exploring the feasibility of combining As(III) and As(V) by oxidizing the former to the latter, to produce a single inorganic As species (iAs). Other objectives will include deciding how many different As species should be captured in the proposed method, while recognizing calibration standards exist only for limited number of As species. A key objective will be to produce a suitable candidate reference material based on a seafood product matrix that can be homogenized, and distributed to multiple laboratories for the first interlaboratory study. Other objectives will include a single laboratory validation of the proposed method, and the design of the first interlaboratory round robin to gather baseline data on network capabilities