Development of a tissue oxygen monitor using near-infrared light with correction for influence of tissue inhomogeneity and quantitaive evaluation of muscle metabolism

开发使用近红外光的组织氧监测仪，校正组织不均匀性的影响并定量评估肌肉代谢

• 批准号: 11558100
• 负责人: YAMAMOTO Katsuyuki
• 金额: $ 8.19万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558100/
• 关键词: near-infrared light; tissue oxygen monitor; muscle; adipose tissue; Monte Carlo simulation; optical properties of tissues; optical imaging

The objectives of this study were to develop a tissue oxygen monitor that can eliminate the influence of a fat layer on muscle oxygenation measurement using near-infrared spectroscopy (NIRS) and to establish quantitative NIRS by verifying the validity of this technique in the field of sports medicine.1. Mean optical properties (absorption and scattering coefficients) of muscle and adipose tissues were determined by comparing results of in vivo measurements using time-resolved NIRS with those of Monte Carlo simulation using a multi-layered model.2. A nonlinear algorithm for tissue oxygenation measurement was theoretically derived, based on diffusion theory, and the coefficients of the algorithm were determined from the mean optical properties of muscles.3. A correction method for eliminating the great influence of a fat layer on measurement sensitivity was developed so that the theoretical algorithm assuming a homogeneous medium could be applied. Validity of this correction method was also confirmed by magnetic resonance spectroscopy.4. As an application of NIRS to exercise physiology, restoration rates of muscle oxygenation after exercise n were measured in 13 top athletes of National Team for Nordic skiing during high-altitude training. It was found that the restoration rates increased by 11 % and 20 % at 6 and 11 days after the beginning of the training, respectively.5. New measurement techniques for estimating muscle metabolism were developed: oxygen saturation of blood in muscles and oxygen consumption of muscles were estimated from change in blood volume and from a decreasing rate of muscle oxygenation, respectively, during contraction.6. A 200-channel imaging system of muscle oxygenation was developed: and spatio-temporal changes in oxy- and deoxy-hemoglobin during knee extension and flexion were successfully imaged on the thigh.

本研究的目的是开发一种组织氧监测仪，可以消除脂肪层对使用近红外光谱（NIRS）测量肌肉氧合的影响，并通过验证该技术在运动医学领域的有效性来建立定量的近红外光谱。肌肉和脂肪组织的平均光学性质（吸收和散射系数）是通过比较使用时间分辨近红外光谱的体内测量结果和使用多层模型的蒙特卡罗模拟结果来确定的。基于扩散理论，从理论上推导了一种用于组织氧合测量的非线性算法，该算法的系数由肌肉的平均光学特性确定。提出了一种消除脂肪层对测量灵敏度影响的修正方法，使假设介质均匀的理论算法得以应用。磁共振波谱分析也证实了该校正方法的有效性。将近红外光谱（NIRS）应用于运动生理学，对13名北欧滑雪国家队优秀运动员在高海拔训练中运动后肌肉氧合恢复率进行了测量。结果发现，在训练开始后的第6天和第11天，恢复率分别提高了11%和20%。研究人员开发了估算肌肉代谢的新测量技术：分别根据收缩过程中血容量的变化和肌肉氧合率的下降来估算肌肉中的血氧饱和度和肌肉的耗氧量。开发了200通道肌肉氧合成像系统，成功地在大腿上成像了膝关节伸展和屈曲期间氧和脱氧血红蛋白的时空变化。

项目成果

Kazuya MAEGAWA: "Influences of overlying tissues on muscle oxygenation measurement using spatially resolved near-infrared spectroscopy"IEICE Technical Report MBE. 200-34. 39-44 (2000)

Kazuya MAEGAWA：“使用空间分辨近红外光谱法测量上覆组织对肌肉氧合的影响”IEICE 技术报告 MBE。

Masaaki AOTA: "Imaging of Invisible: Visuaization techniques and applications"Journal of IEICE. 83-9. 675-680 (2000)

Masaaki AOTA：“不可见的成像：可视化技术和应用”IEICE 杂志。

Masatsugu NIWAYAMA: "Development of 200-channel mapping system for tissue oxygenation measured by near-infrared spectroscopy (Invited Paper)"Proceedings of SPIE. 4082. 48-56 (2000)

Masatsugu NIWAYAMA：“开发近红外光谱测量组织氧合的 200 通道绘图系统（特邀论文）”SPIE 论文集。

Masatsugu NIWAYAMA: "Quantitative measurement of muscle hemoglobin oxygenation using near-infrared spectroscopy with correction for the influence of a subcutaneous fat layer"Rev.Sci.Instrum.. 71(12). 4571-4575 (2000)

Masatsugu NIWAYAMA：“使用近红外光谱法定量测量肌肉血红蛋白氧合，并校正皮下脂肪层的影响”Rev.Sci.Instrum.. 71(12)。

前川和也: "空間分解近赤外分光法を用いた筋組織酸素濃度計測における介在組織の影響"電子情報通信学会技術研究報告. MBE2000-34. 39-44 (2000)

Kazuya Maekawa：“使用空间分辨近红外光谱测量介入组织对肌肉组织氧浓度的影响”MBE2000-34 (2000)。