Development of an instrument for temporal and spatial analyses of distribution of muscle oxygenation using near-infrared spectroscopy and its applications

近红外光谱肌肉氧合分布时空分析仪器的研制及其应用

• 批准号: 16300142
• 负责人: YAMAMOTO Katsuyuki
• 金额: $ 9.09万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300142/
• 关键词: near-infrared light; skeletal muscle; muscle oxygenation; oxygen consumption rate; temporal and spatial analyses; exercise tests; microspectrophotometry; 組織酵素濃度

In this study, a small and lightweight instrument based on spatially resolved near-infrared spectroscopy (SR-NIRS) was developed for real-time imaging of muscle oxygenation. From the results of temporal and spatial analyses of muscle oxygenation during exercise tests, we elucidated the relationship between changes in muscle oxygenation and metabolism and established the basics of NIRS as a standard technique for functional imaging of muscle. The following results were obtained.1. Development of an imaging instrument for muscle oxygenation based on spatially resolved NIRSCompact multi-channel optical probes with sufficient S/N were constructed. The measurement reliability of the probes was validated using a liquid tissue-mimic phantom experiment (blood and Intralipid). In addition, instrumentation control, data acquisition and user-interface programs were coded. The data acquisition time when 32 probes (3 channel X 32 probes = 96 channels) were used, including time for calculation of ox … More ygenation, was about 500 ms. Therefore, real-time imaging of instantaneous changes in muscle oxygenation during exercise is possible.2. Temporal and spatial analyses during exercise tests using human subjectsThe developed instrument was used to analyze temporal and spatial changes in tissue oxygen saturation (TOS) in response to different exercises. Spatial and temporal differences in TOS of the quadriceps muscle during exercise tests with small modifications in exercise protocol (e.g., knee extension exercise without or with leg press action) were detected. In addition, the influence of oxygen debt on oxygen consumption rate ( Vo2) of the tibialis anterior muscle during repeated exercise (two similar protocols) was investigated, with comparison of Vo2 during the former and latter exercises. Due to oxygen debt, the VO2 values of the latter exercise are a direct summation of those of the former exercise if TOS did not decrease significantly during exercise.3. Measurement of oxygen saturation of muscle using micro-spectroscopyA micro-spectroscopy system that uses (i) light in the wavelength range of 400 to 850 nm to measure optical density (light attenuation) and (ii) multiple regression analysis to eliminate the scattering component was constructed. Using this system, imaging of oxygen saturation of the cremaster muscle of a rat was performed. Oxygen saturation of blood vessels with radii of about 10 μm can be sufficiently imaged using wavelengths around 500 nm. However, shorter wavelengths (400 to 500 nm range) are needed in order to image oxygen saturation of blood vessels with smaller radii.4. Other techniques for utilizing SR-NIRS in practical applicationsA highly-sensitive CCD camera was used in place of the multi-channel optical probes described above to validate the appropriateness of the solution of diffusion approximation for a highly scattered medium, in which SR-NIRS is based on. In addition, solid plastic phantoms which are easy to handle and stable over a long period, with optical properties matched to those of human tissue over a range of near-infrared wavelengths, were constructed. It is expected that these phantoms will be used in the future to calibrate imaging instruments designed for practical applications in sports and rehabilitation medicine. Less

在这项研究中，一个小而轻的仪器的基础上，空间分辨近红外光谱（SR-NIRS）的肌肉氧合实时成像。从运动试验过程中肌肉氧合的时间和空间分析结果，我们阐明了肌肉氧合和代谢变化之间的关系，并建立了近红外光谱作为肌肉功能成像的标准技术的基础。得到以下结果.基于空间分辨近红外光谱仪的肌肉氧合成像仪的研制构造了具有足够信噪比的紧凑型多通道光学探头。使用液体组织模拟体模实验（血液和胰岛素）验证探头的测量可靠性。此外，仪器控制，数据采集和用户界面程序的编码。使用32个探头（3通道X 32探头= 96通道）时的数据采集时间，包括计算ox的时间 ...更多信息 因此，实时成像运动过程中肌肉氧合的瞬时变化是可能的.利用自行研制的运动试验仪，分析了不同运动条件下人体组织氧饱和度（TOS）的时空变化。在运动试验期间，对运动方案进行小的修改（例如，没有或有压腿动作的膝伸展运动）。此外，还研究了重复运动（两种相同的运动方案）中氧债对胫骨前肌耗氧率（Vo 2）的影响，并比较了前、后两种运动中的Vo 2。由于氧债的存在，如果运动过程中TOS没有显著降低，则后一种运动的VO 2值是前一种运动的VO 2值的直接总和.使用显微分光光度计测量肌肉的氧饱和度构建了显微分光光度计系统，其使用（i）400至850 nm波长范围内的光来测量光密度（光衰减）和（ii）多重回归分析来消除散射分量。使用该系统，进行大鼠提睾肌的氧饱和度成像。半径约为10 μm的血管的氧饱和度可以使用500 nm左右的波长充分成像。然而，需要更短的波长（400至500 nm范围）以便对具有更小半径的血管的氧饱和度成像。在实际应用中使用SR-NIRS的其他技术使用高灵敏度CCD相机代替上述多通道光学探针，以验证SR-NIRS所基于的高度散射介质的扩散近似解的适当性。此外，固体塑料体模易于处理且长期稳定，其光学特性在近红外波长范围内与人体组织的光学特性相匹配。预计这些幻影将在未来用于校准成像仪器设计的运动和康复医学的实际应用。少

项目成果

NIRS測定値解釈のための筋代謝動態のシミュレーション : 運動負荷に対する定常及び過渡応答

模拟肌肉代谢动力学以解释 NIRS 测量结果：对运动负荷的稳定和瞬态响应

• 发表时间: 2004
• 期刊: 電気情報通信学会技術研究報告 104(129)
• 作者: Khai Jun KEK;宮川 崇拓;Takahiro MIYAKAWA;Khai Jun Kek;Khai Jun Kek;山本克之;Kengo OKADA;Khai Jun KEK;Kengo Okada;Satoshi Yamada;Nobuki Kudo;Khai Jun KEK;米山聡子
• 通讯作者: 米山聡子

近赤外分光法による運動時の筋組織局所酸素消費量の測定

使用近红外光谱测量运动过程中肌肉组织的局部耗氧量

• 发表时间: 2006
• 期刊: 電子情報通信学会技術研究報告 106(127)
• 作者: Khai Jun KEK;宮川 崇拓
• 通讯作者: 宮川 崇拓

Functional imaging of muscle oxygenation and oxygen consumption in the knee extensor muscles during isometric contractions by spatially resolved near-infrared spectroscopy

通过空间分辨近红外光谱对等长收缩期间膝伸肌的肌肉氧合和耗氧量进行功能成像

• 发表时间: 2000
• 期刊: Proceedings of SPIE 6434(in press)
• 作者: Khai Jun KEK;宮川 崇拓;Takahiro MIYAKAWA;Khai Jun Kek;Khai Jun Kek;山本克之;Kengo OKADA;Khai Jun KEK;Kengo Okada;Satoshi Yamada;Nobuki Kudo;Khai Jun KEK;米山聡子;前川耕太;Khai Jun KEK;Nobuki KUDO;Satoko YONEYAMA;Koto MAEKAWA;Nobuki KUDO;米山聡子;Nobuki KUDO;Khai Jun KEK
• 通讯作者: Khai Jun KEK

A basic study on sonoporation with microbubbles exposed to pulsed ultrasound.

• DOI: 10.1007/s10396-005-0031-5
• 发表时间: 2005-03-01
• 期刊: Journal of medical ultrasonics (2001)
• 作者: Okada, Kengo;Kudo, Nobuki;Yamamoto, Katsuyuki
• 通讯作者: Yamamoto, Katsuyuki

Measurement of change in local muscle oxygen consumption during exercise using near-infrared spectroscopy

使用近红外光谱测量运动过程中局部肌肉耗氧量的变化

• 发表时间: 2006
• 期刊: Technical Report of IEICE 106(127)
• 作者: Khai Jun KEK;宮川 崇拓;Takahiro MIYAKAWA
• 通讯作者: Takahiro MIYAKAWA