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Molecular epidemiology of rabies and lyssaviruses infection in Southeast Asia

东南亚狂犬病和狂犬病病毒感染的分子流行病学

基本信息

批准号：
11691181
负责人：
MINAMOTO Nobuyuki
金额：
$ 1.98万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691181/
关键词：
Rabies virus Lyssa viruses Southeast Asia Molecular epidemiology N gene RFLP Dog Bat 犬野ネズミ cDNA

项目摘要

In this study, to definite the infectious movement of rabies virus in animals and the distribution of lyssaviruses in wildlifes in Southeast Asia, brain samples of rabid animals, bats and wild rats were collected from enzootic areas of Thailand and Indonesia. Total RNA from brains was extracted, reverse-transcribed, amplified by PCR (RT-PCR), directly sequenced and analyzed for N and G genes.The results run as follows :1) All 61 samples from several areas of Thailand were separated into at least six N-genotypes by the sequencing and RFLP analyses. Of them, four genotypes indicated a good correlation between genetic and geographical criteria, and the remaining genotypes were overlap between two areas.2) The N-genotype of rabies isolates from the north-eastern area was similar to it of other regions but not idenntified in detail.3) A comparative analysis of G gene in rabies isolates from several regions of Thailand, revealed that the deduced amino acid sequences of the G proteins had a high homology (97-98%) among each other, but were 88-89% with the RC-HL strain, which is presently used for vaccine for dog in Japan.4) All samples of 50 wild rats and 50 bats from the south area in Thailand were negative for the detection of lyssavirus N gene by RT-PCR.5) At present, genetic analysis of 140 rabies isolated collected from several areas in Indonesia is the progressing.

为了明确狂犬病病毒在东南亚地区动物体内的感染动态和狂犬病病毒在野生动物体内的分布，本研究从泰国和印度尼西亚的狂犬病流行区采集了狂犬病动物、蝙蝠和野鼠的脑组织样品。结果表明：（1）从泰国不同地区采集的61份样品经测序和RFLP分析，至少可分为6种N基因型。结果表明：（1）泰国东北部地区狂犬病分离株的N基因型与其他地区狂犬病分离株的N基因型相似，但未详细区分;（2）泰国东北部地区狂犬病分离株的G基因型与其他地区狂犬病分离株的N基因型相似，但未详细区分;（3）泰国东北部地区狂犬病分离株的G基因型与其他地区狂犬病分离株的G基因型相似，但未详细区分。结果表明，G蛋白的氨基酸序列相互之间有很高的同源性（97-98%），但与RC-HL株的同源性为88-89%，目前在日本用于犬用疫苗。4）来自泰国南部地区的50只野鼠和50只蝙蝠样品，用RT-PCR检测狂犬病毒N基因均为阴性。5）目前，从印度尼西亚几个地区收集的140个狂犬病分离株的遗传分析正在进行中。