A role as a zoonotic disease-causing agent of rotavirus

为了明确轮状病毒作为人畜共患致病因子的参与，从与人密切接触的几种动物中分离出病毒，并对其病毒进行了抗原和遗传分析。病毒的分离是在许多动物物种中进行的，包括狗、猫和宠物鸟，但与我们的预期相反，因此只从狗中分离出三株病毒。然后，对从鸽子中分离的PO-13株进行了抗原性和遗传特性的测定，以明确鸽子与人轮状病毒之间的关系。利用间接免疫荧光抗体法对PO-13菌株进行VP6的单克隆抗体亚群鉴定。在29个单抗中，5个单抗仅与具有I亚群抗原的轮状病毒反应。因此，发现PO-13株具有I亚群抗原。通过与7种不同血清型轮状病毒的交叉中和试验，将PO-13毒株确定为血清型7，与在北爱尔兰恢复的火鸡轮状病毒更相似。在病毒dsRNA的聚丙烯酰胺凝胶电泳中，鸽轮状病毒与哺乳动物轮状病毒之间最显著的差异是dsRNA第5、10和11段的电泳迁移。PO-13毒株第5段的迁移速度比哺乳动物轮状病毒慢，第10段和第11段的迁移非常紧密。4种不同血清型人轮状病毒与^<32> p标记的PO-13探针之间的RNA-RNA杂交条带仅在同源反应中观察到。上述结果表明，PO-13菌株在血清型和基因型上与代表血清型1-4的原型人菌株不完全相关。包括本研究结果和先前报道的在许多人体内检测到PO-13株血凝抑制抗体的结果，表明与PO-13株具有抗原相似性的未知轮状病毒广泛存在于人类和动物群体中。目前正在对从几只伴侣动物身上分离出的轮状病毒进行进一步调查。少

As a clue for clarifying a participation as a zoonotic disease-causing agent of rotavirus, viral isolation from several animals in close contact with human , and antigenic and genetic analysis of its virus were performed. The isolation of virus was carried out in a number of animal species, including, dogs, cats and pet birds, but contrary to our expectations, so for only three strains were isolated from dogs. Then, antigenic and genetic properties of the PO-13 strain isolated from pigeon were determined to clear relationship between pigeon and human rotaviruses.An attempt to subgroup the PO-13 strain made by indirect immunofluorescent antibody assay used monoclonal antibodies (MAbs) to its VP6. Of 29 MAbs, 5 reacted only with rotaviruses possessed subgroup I antigen. Therefore, It was found that the PO-13 strain has subgroup I antigen. By the cross neutralization test examined between the PO-13 strain with 7 different serotypes of rotavirus, the PO-13 strain was designated serotype 7 … More similarly with turkey rotavirus recovered in Northern Ireland. In the polyacrylamide gel electrophoresis of viral dsRNA, the most striking difference between the pigeon and mammalian rotaviruses is indicated the electrophoretic migration of dsRNA segments 5, 10 and 11. Segment 5 of PO-13 strain migrates more slowly than those of mammalian rotaviruses, and segments 10 and 11 migrate very closely. RNA-RNA hybrid bands obtained between 4 different serotypes of human rotaviruses and ^<32>P-labeled PO-13 probe were observed only in the homologous reaction.These results indicated that the PO-13 strain is not entirely related in serotype and genotype to the prototype human strain representing serotypes 1-4. The findings including the present results and previously reports which hemagglutination inhibition antibody to the PO-13 strain was detected in many human, would suggests the fact that unknown rotavirus resembled antigenically with the PO-13 strain was widespread human and animal populations. Further investigations on the rotavirus isolation from several companion animals are now in progress. Less