权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A role as a zoonotic disease-causing agent of rotavirus

轮状病毒作为人畜共患疾病病原体的作用

基本信息

批准号：
63560302
负责人：
MINAMOTO Nobuyuki
金额：
$ 1.15万
依托单位：
Gifu University, Faculty of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

As a clue for clarifying a participation as a zoonotic disease-causing agent of rotavirus, viral isolation from several animals in close contact with human , and antigenic and genetic analysis of its virus were performed. The isolation of virus was carried out in a number of animal species, including, dogs, cats and pet birds, but contrary to our expectations, so for only three strains were isolated from dogs. Then, antigenic and genetic properties of the PO-13 strain isolated from pigeon were determined to clear relationship between pigeon and human rotaviruses.An attempt to subgroup the PO-13 strain made by indirect immunofluorescent antibody assay used monoclonal antibodies (MAbs) to its VP6. Of 29 MAbs, 5 reacted only with rotaviruses possessed subgroup I antigen. Therefore, It was found that the PO-13 strain has subgroup I antigen. By the cross neutralization test examined between the PO-13 strain with 7 different serotypes of rotavirus, the PO-13 strain was designated serotype 7 … More similarly with turkey rotavirus recovered in Northern Ireland. In the polyacrylamide gel electrophoresis of viral dsRNA, the most striking difference between the pigeon and mammalian rotaviruses is indicated the electrophoretic migration of dsRNA segments 5, 10 and 11. Segment 5 of PO-13 strain migrates more slowly than those of mammalian rotaviruses, and segments 10 and 11 migrate very closely. RNA-RNA hybrid bands obtained between 4 different serotypes of human rotaviruses and ^<32>P-labeled PO-13 probe were observed only in the homologous reaction.These results indicated that the PO-13 strain is not entirely related in serotype and genotype to the prototype human strain representing serotypes 1-4. The findings including the present results and previously reports which hemagglutination inhibition antibody to the PO-13 strain was detected in many human, would suggests the fact that unknown rotavirus resembled antigenically with the PO-13 strain was widespread human and animal populations. Further investigations on the rotavirus isolation from several companion animals are now in progress. Less

为阐明轮状病毒作为人畜共患病病原体的参与，对几种与人密切接触的动物进行了病毒分离，并对其病毒进行了抗原性和基因分析。在许多动物物种中进行了病毒分离，包括狗、猫和宠物鸟，但与我们的预期相反，只从狗身上分离出三种毒株。为了明确鸽轮状病毒与人轮状病毒的亲缘关系，对分离自鸽的PO-13毒株进行了抗原性和遗传特性的测定，并尝试用抗人轮状病毒VP6的单抗对用间接免疫荧光方法制备的PO-13毒株进行亚群划分。在29株单抗中，有5株仅与具有I亚群抗原的轮状病毒反应。由此可见，PO-13株具有I亚群抗原。通过PO-13株与7种不同血清型轮状病毒的交叉中和试验，将PO-13株命名为7型…更类似的是，在北爱尔兰发现了火鸡轮状病毒。在病毒dsRNA的聚丙烯酰胺凝胶电泳图中，鸽子轮状病毒和哺乳动物轮状病毒最显著的区别是dsRNA片段5、10和11的电泳性迁移。PO-13株的5片段迁移速度慢于哺乳动物轮状病毒，10和11片段迁移非常紧密。4种不同血清型的人轮状病毒与~(32)&gt；P标记的PO-13探针的RNA-RNA杂交带仅在同源反应中观察到，提示PO-13株与代表血清型1~4型的原型人株在血清型和基因型别上并不完全相关。这些发现包括目前的结果和以前在许多人中检测到PO-13株的血凝抑制抗体的报道，表明未知的轮状病毒与PO-13株在抗原性上相似这一事实在人和动物中广泛存在。从几只同伴动物身上分离出轮状病毒的进一步研究目前正在进行中。较少