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Molecular epidemiology of hepatitis viruses in Indonesia

印度尼西亚肝炎病毒的分子流行病学

基本信息

批准号：
11691209
负责人：
HOTTA Hak
金额：
$ 4.74万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691209/
关键词：
Indonesia hepatitis C virus GB virus TT virus hepatitis B virus hepatitis D virus molecularepidemiology viremia titer 遺伝子型多様性

项目摘要

Molecular epidemiological analysis was performed on hepatitis C virus (HCV), hepatitis B virus (HBV), hepatitis D virus (HDV) and possible hepatitis virus candidates, GB virus (GBV) and TT virus (TTV), in Surabaya, Indonesia.1. HCV.Mean HCV viremia titers of HCV-1b and HCV-1c were practically the same, and significantly higher than that of HCV-2a. Even among the same subtype, however, HCV viremia titers varied considerably with different HCV isolates. Sequence analysis of HCV revealed that HCV viremia titers were significantly lower when the number of amino acid mutations in the interferon sensitivity determining region (ISDR) of NS5A was four or more, compared with isolates having ISDR mutations of three or less. No other particular mutation (s) was identified in NS5A that correlated well with HCV viremia titers.2. GBV.The prevalence of serum GBV RNA was 2.7% in blood donors, 5.7% in chronic hepatitis patients, 11.5% in liver cirrhosis patients, 7.0% in hepatocellular carcinoma patien … More ts and 29.0% in hemodialysis patients in Surabaya, Indonesia. GBV posicivity was significantly higher in anti-HCV-positive blood donors than in anti-HCV-negative blood donors. No significant correlation was observed between GBV positivity and abnormal liver function tests, suggesting non-hepatopathogenic features of GBV.Phylogenetic analysis identified a novel genotype of GBV (type 5), which represented 40% of total GBV in Surabaya, Indonesia.3. TTV.The prevalence of serum TTV DNA was 33% in blood donors, 22% in chronic hepatitis patients, 24% in liver cirrhosis patients, 46% in hepatocellular carcinoma patients and 44% in hemodialysis patients in Surabaya, Indonesia. Those TTV isolates were phylogenetically classified into Gla, Glb and an as-yet-unclassifiable genotype.4. HBV.All of HBV isolates in Surabaya, Indonesia, were phylogenetically classified into genotype B.5. HDV.HDV was shown to be rather rare in Surabaya, Indonesia, with the prevalence of anti-HDV antibodies being less than 0.5%. Less

对印尼泗水地区的丙型肝炎病毒(丙型肝炎病毒)、乙型肝炎病毒(乙肝病毒)、丁型肝炎病毒(HDV)以及可能的候选肝炎病毒GB病毒(GBV)和TT病毒(TTV)进行了分子流行病学分析。丙型肝炎病毒1b和丙型肝炎病毒1c的平均病毒血症滴度基本相同，显著高于丙型肝炎病毒2a的滴度。然而，即使在相同的亚型中，不同的丙型肝炎病毒分离株的病毒血症滴度也有很大的不同。对丙型肝炎病毒的序列分析显示，当NS5A干扰素敏感决定区(ISDR)的氨基酸突变数为4个或更多时，与ISDR突变为3个或更少的分离株相比，丙型肝炎病毒血症滴度显著降低。未发现NS5A中其他与丙型肝炎病毒病毒血症滴度有很好相关性的特殊突变(S)。献血员血清GBVRNA阳性率为2.7%，慢性肝炎组为5.7%，肝硬化组为11.5%，肝细胞癌组为7.0%印尼苏腊巴亚的血液透析患者中TS和29.0%更多。抗-丙型肝炎病毒阳性献血员的GBV阳性率显著高于抗-丙型肝炎病毒阴性献血员。未发现GBV阳性与肝功能异常试验之间的显著相关性，提示GBV的非肝病特征。系统发育分析发现了一种新的GBV基因型(5型)，占印尼泗水地区GBV总数的40%。印尼泗水的献血员、慢性肝炎患者、肝硬变患者、肝细胞癌患者和血液透析患者的血清TTV DNA的患病率分别为33%、22%、24%、46%和44%。TTV分离株在系统发育上分为GLA、GLB型和尚未分类的GLB型。所有印度尼西亚苏腊巴亚的乙肝病毒分离株都被系统发育分类为B.5型。HDV在印度尼西亚的泗水相当罕见，抗HDV抗体的流行率不到0.5%。较少