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Application of a color-center laser to an immunoassay using an IR-Raman spectroscopy technique.

色心激光器在使用红外拉曼光谱技术的免疫测定中的应用。

基本信息

批准号：
11694115
负责人：
KAMEMARU Shun-ichi
金额：
$ 4.35万
依托单位：
Kitami Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Results from the Scientific Research (B) (2) during the term of the project are as follows ;1 Results from C-F bond mapping (10 micron) by a Microscopic FT-IR spectroscopy ;(1) Development of protocol for inducing a fluorine-containing substituent to an antibody : synthesis of a complex dye and its application to wide pH range measurement.(2) Continuous discussion of a modified antibody with fluorine for application to an immunoassaymodel reaction : quantitative reaction of the proposed dye with amino group of protein is observed. However, the application of this technique to an immunity model has not been accomplished due to impossibility of enormous amount of synthesis of the dye.2 Selection of a laser for a spectrum source and its application to IR observation ;(1) Green light is the best as a light source for an IR-measurement system using the proposed dye, which showed both a white light laser (by Prof. Fujii) and a color center laser (by Prof. Mirov) are suitable for the light source.3 A high resolution (1-micron) image is obtained by the IR laser Raman microscope ;(1) Using a very accurate Raman mapping technique, possibility of 3-D mapping of the surface of cells modified with fluorine-containing dye is achieved.Further research in the next project : During the experiments in the project, the proposed technique using an IR laser Raman microscope to obtain a C-F bond absorption image is found to be suitable for obtaining data of non-bio materials as well as bio-materials. We hope to try, for example, to develop a material for a set of artificial teeth without using an antibacterial agent for elimination of bacterial.

在项目期间，科学研究（B）（2）的结果如下：1通过显微FT-IR光谱进行的C-F键图谱（10微米）的结果;（1）开发用于诱导抗体含氟取代基的方案：合成复合染料及其在宽pH范围测量中的应用。(2)继续讨论用氟修饰的抗体应用于免疫测定模型反应：观察到所提出的染料与蛋白质的氨基的定量反应。然而，由于染料的大量合成是不可能的，因此该技术在免疫模型中的应用尚未完成。2光谱源激光器的选择及其在红外观测中的应用;（1）绿色光作为使用所提出的染料的IR测量系统的光源是最好的，显示了一束白色激光（藤井教授）和色心激光器（作者：Mirov教授）适用于光源。3高分辨率通过IR激光拉曼显微镜获得（1微米）图像;（1）使用非常精确的拉曼映射技术，实现了含氟染料修饰的细胞表面的三维绘图的可能性。下一个项目的进一步研究：在实验过程中，我们发现利用红外激光拉曼显微镜获得C-F键吸收图像的方法既适用于生物材料，也适用于非生物材料。例如，我们希望尝试开发一种不使用抗菌剂的人造牙齿材料，以消除细菌。