Application of a whitelight laser and dye to medical treatment.

白光激光和染料在医疗中的应用。

• 批准号: 09044116
• 负责人: KAMEMARU Shun-ichi
• 金额: $ 4.99万
• 依托单位: Kitami Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044116/
• 关键词: Immunoassay; Automated measurement of cell; Fluorescent dye; Cytokin; Image processing; Fluorine-containing compound; White light laser

Results from the International Scientific Research, Joint Research, during 1998 are as follows ;1) Automated observation of cells : Auto-focus mechanism and auto-scanning of the cell stage systems were developed. Manually adjusting systems were improved to automated ones by the technique using a feedback control method through observed image signals.2) Preparation of new fluorescent dyes and development of staining protocol : Quantitative reaction of trifluoroethyl esters of fluorine-containing carboxylic acids to proteins was found. Microscopic infrared mapping of carbon-fluorine bonds could beutilized complementarily with ILISPOT assay. These results are only realized by a prototype system, however, those would soon be realized by a clinical inspection system.3) Processing of the observed imaging signals : Recognition technique for identifying pseudo-spot with uniform intensity from real spot were developed using soft ware algorithm. Using this technique, high S/N ration signals were obtained without changing the hardware part of the proposed system.4) Discussion of a laser as a light source : Throughout theoretical discussions and simulations, the idea of 40% down sizing of the conventional laser was proposed as a new illuminating light source for the smaller system in a clinical field.^<**>Further research in the next project : During the experiments in this project, Dr.Mirov, investigator, found that tomographic imaging of the cells could be realized using an illumination by a tunable wavelength laser. Realization of the system, called a laser Raman microscope, has great possibility for an application to an Immunoassay System in the near future.

1998年国际科学研究联合研究的结果如下；1）细胞的自动观察：开发了自动聚焦机制和自动扫描细胞阶段系统。通过观察图像信号，采用反馈控制方法，将手动调节系统改进为自动调节系统。2)新型荧光染料的制备和染色方案的开发：发现了含氟羧酸的三氟乙酯与蛋白质的定量反应。碳-氟键的显微红外图谱可以与 ILISPOT 测定互补使用。这些结果只能通过原型系统实现，但是，这些结果很快就会通过临床检查系统实现。3）观察到的成像信号的处理：使用软件算法开发了用于从真实点中识别强度均匀的伪点的识别技术。使用这种技术，在不改变所提出系统的硬件部分的情况下获得了高信噪比信号。4) 激光作为光源的讨论：通过理论讨论和模拟，提出了将传统激光尺寸缩小 40% 的想法，作为临床领域较小系统的新照明光源。^<**>下一个项目中的进一步研究：在该项目的实验过程中，研究员 Mirov 博士发现 使用可调谐波长激光的照明可以实现细胞的断层成像。该系统的实现称为激光拉曼显微镜，在不久的将来很有可能应用于免疫分析系统。

项目成果

S.B.Mirov and A.Yu.Dergachev: "Efficient room temperature LiF : F_2^<+**> color center tunable laser tunable over 820-1210 nm range." Optics Comm.145. 107-112 (1998)

S.B.Mirov 和 A.Yu.Dergachev：“高效室温 LiF：F_2^< **> 色心可调谐激光可在 820-1210 nm 范围内调谐。”

McGhee, J.R., Kiyono, H., Kubota, M., Kawabata, S., Miller, C.J., Lehner, T., Imaoka, K.and Fujihashi, K.: "Mucosal Th1-versus Th2-type responses for antibody or cell mediated immunity to SIV rhesus macaques." J.Infect.Dise.(in press).

McGhee, J.R.、Kiyono, H.、Kubota, M.、Kawabata, S.、Miller, C.J.、Lehner, T.、Imaoka, K. 和 Fujihashi, K.：“抗体或药物的粘膜 Th1 与 Th2 型反应

Kubota, T.: "EPC-synthesis of Fluoro-Organic Compounds : Stereochemical Challenges and Biomedical Targets edited by Vadiam A. Soloshonok" John Wiley & Sons, Ltd., 740 (1998)

Kubota, T.：“含氟有机化合物的 EPC 合成：立体化学挑战和生物医学目标，由 Vadiam A. Soloshonok 编辑”John Wiley

Kiyono, H.: "Inflammatory Bowel Deseases and Recurrent Abdominal Pain in Chldren" Kluwer Academic publishers, 350 (1997)

Kiyono, H.：“儿童炎症性肠病和复发性腹痛”Kluwer 学术出版社，350 (1997)

S.B.Mirov: "Efficient room temperature LiF : F_2^<+**> color center tunable laser tunable over 820- 1210 nm range" 0ptics Comm.Vol.145. 107-112 (1998)

S.B.Mirov：“高效室温 LiF：F_2^< **> 色心可调谐激光可在 820-1210 nm 范围内调谐”0ptics Comm.Vol.145。