REGULATION OF OSTEOCLASTIC BONE RESORPTION BY RHO

RHO 对破骨性骨吸收的调节

基本信息

批准号：
11694283
负责人：
MORIYAMA Keiji
金额：
$ 2.05万
依托单位：
THE UNIVERSITY OF TOKUSHIMA
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

At the time when a mechanical force is applied onto the tooth, mechanical stress is loaded on the periodontal tissues. A number of osteocytes are located in the bone matrix, communicating each other, as well as with the osteoblasts on the bone surface, via cytoplasmatic processes and gap junctions. Such a cellular network is speculated to have certain important functions for the adaptation to a mechanical loaded environment by the detection of mechanical stress.In the present study, in order to investigate the role of osteocytes in bone resorption, we examined the effects of purified chick osteocyte-derived protein (ODP) and Rho-GTP-dissociation inhibitor (Rho-GDI) on bone resorption. Recently, we established a culture system which provided with human mature osteoclasts from peripheral mononuclear blood cells (PMBC) by using M-CSF and human soluble RANKL (sRANKL) in the absence of osteoblasts and stromal cells. FITC-labelled alpha-actinin pre-microinjected into human osteoclasts was in … More corporated into podosomes which lined the periphery of the cells. Although the podosomes were still observed after the injection of buffer, microinjection of ODP and Rho-GDI caused disruption of podosomes within 30 min of injection. Furthermore, both proteins markedly inhibited pit formation, whereas the conditioned medium of osteocytes had no effect.This study has also focused on a novel cysteine proteinase, cathepsin K, which is through to be specifically expressed in osteoclasts and plays an important part in osteoclastic bone resorption. We have investigated the mechanism of alveolar bone remodelling during orthodontic tooth movement based on an examination of changes in cathepsin K mRNA expression in parallel with the histological changes in alveolar bone.Therefore, further insights into osteoclasts differentiation and function mechanisms may provide stronger bases that will help to understand with more detail the fundaments of orthodontic tooth movement and the reaction of mechanically stressed periodontal tissues. Less

在将机械力施加到牙齿上时，将机械应力加载到牙周组织上。许多骨细胞位于骨基质中，相互交流，并通过细胞质过程和间隙连接处与骨表面上的成骨细胞交流。推测这种细胞网络具有通过检测机械应力适应机械负荷环境的某些重要功能。在本研究中，为了研究骨细胞在骨骼分辨率中的作用，我们检查了纯化的雏鸡骨化细胞骨细胞骨细胞衍生的蛋白（ODP）和rhO-GTPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPP的影响。最近，我们建立了一个培养系统，该系统在没有成骨细胞和基质细胞的情况下，使用M-CSF和人类可溶性Rankl（Srankl）提供了外周单核血细胞（PMBC）的人类成熟破骨细胞。在人类破骨细胞中的FITC标记的α-肌动蛋白预注射的是……更多的企业中的足体成衬有细胞周围的足体。尽管在注射缓冲液后仍观察到足体，但在注射后30分钟内，ODP和Rho-GDI的显微注射导致了足体的破坏。此外，两种蛋白质都显着抑制了凹坑的形成，而条件骨细胞的条件培养基无效。这项研究还集中在一种新型半胱氨酸蛋白酶calterpsin K上，该蛋白酶K始终在破骨细胞中特异性表达，并在整骨碎裂骨骼分辨率中起重要作用。我们已经根据对牙髓骨骨的组织学变化并行的检查，研究了正畸牙齿运动过程中牙槽骨重塑的机理。因此，对破骨细胞分化和功能机制的进一步见解可能会提供强大的基础，从而有助于更多地了解正畸牙齿运动的基础以及机械压力的牙周组织的反应。较少的