Hormonal and pheromonal control of reproduction

生殖的激素和信息素控制

基本信息

批准号：
12440234
负责人：
KIKKUYAMA Sakae
金额：
$ 9.86万
依托单位：
Waseda university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Hormonal and pheromonal control of reproduction was investigated focusing on the hormones and pheromones involved in the expression of reproductive behavior in the newt, Cynopus pyrrhogaster. During the breeding season, the male newt exhibit a specific courtship behavior; vibration of the tail in front of the female partner discharge of a female-attracting pheromone (sodefrin) and deposition of spermatophores. The tail vibration was revealed to be induced by prolactin (PRL) and androgen. The expression of this behavior was enhanced by arginine vasotocin (AVT) via V la receptor. In this case, PRL and AVT were evidenced to act centrally. In the case of PRL, the site of action was postulated to be neurons situated in the preoptic recess organ and / or nucleus infundibularis dorsalis where immunoreactive PRL receptors are abundant AVT also induced the discharge of both sodefrin and spermatophore presumably by acting peripherally. Synthesis of sodefrin precursor protein in the abdominal gland of the cloaca was considered to be enhanced by PRL and androgen since sodefrin precursor mRNA levels were elevated by PRL and androgen. Responsiyeness of the vomeronasal epithelium of the female newt to sodefrin was also revealed to be under the control of PRL and estrogen as judged from electroolfactogram response. Sodefrin-precursor-like protein cDNA was obtained from a cDNA library of C. ensicauda abdominal gland. According to the deduced amino acid sequence of the protein, sodefrin-like pheromone of C. ensicauda was considered to be decapeptide with a substitution of two amino acid residues. This putative pheromone was isolated from the abdominal gland of C. ensicauda and presence of its bioactivity was confirmed. The pheromone, designated silefrin, was effective only on the conspesific female, as in the case of sodefrin, suggesting the newt pheromones are species-specifia.

研究了繁殖的激素和信息素控制，重点介绍了与纽特cynopus pyrrhogaster中涉及生殖行为表达的激素和信息素。在繁殖季节，男性纽特表现出特定的求爱行为；女性吸引信息素（Sodefrin）和精子下沉积的女性伴侣出院前尾巴的振动。尾部振动显示是由催乳素（PRL）和雄激素诱导的。精氨酸血管素（AVT）通过V LA受体增强了这种行为的表达。在这种情况下，PRL和AVT被证明是集中行动的。在PRL的情况下，假设作用部位是位于前凹槽器官和 /或iNfundundibularis背核中的神经元，其中免疫反应性PRL受体是丰富的AVT，也诱导了Sodefrin和Spermatophore的排出，可能是通过外周的外围表现出来的。由于Sodefrin前体mRNA水平通过PRL和雄激素升高，因此认为泄殖腔的腹膜中Sodefrin前体蛋白的合成被认为可以增强。还揭示了雌性Newt对Sodefrin的呕吐上皮的责任感在PRL和雌激素的控制之下，这是从电载术反应中所判断的。 Sodefrin-Ecursor样蛋白cDNA是从C. Ensicauda腹膜的cDNA库中获得的。根据推导的蛋白质氨基酸序列，C。C。sodefrin样信息素被认为是脱肽，并取代了两个氨基酸残基。从C. ensicauda的腹膜中分离出这种推定的信息素，并证实了其生物活性的存在。信息素（指定为silefrin）仅对同情女性有效，例如Sodefrin，这表明Newt信息素是物种特异性的。