Development of detection system by using infection of Cryptosporidium purvum oocyst to cultured human cell line

浅色隐孢子虫卵囊感染人培养细胞系检测系统的研制

• 批准号: 12450214
• 负责人: ONO Yoshio
• 金额: $ 8.32万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450214/
• 关键词: Cryptosporidium purvum; ultra violet; infection; cell culture; ELISA; 脱嚢能; 消毒; 紫外線; クリプトスポリジウム; 小腸細胞; ELISA

Cryptosporidium purvum is infectious microbes thorough drinking water. There are some detection methods for its low concentration such as de-spored counting. In this research, to develop more convenient system, the cell culture was applied as a carrier tissue. The cell was cultured from human small intestine cancer cell, HCT-8. Cryptosporidium could infect that was detected by ELISA method.As a purpose of disinfection of the organism, we focused its infectious potential to the cells. Living-cells and Fixed-cell culture showed the difference between infection and normal cell. Ultra violet was found to be effective to decrease the infectious potential of the organism. The intensity of the UV was 20 mW sec/cm^2.

purvum隐孢子虫是一种通过饮用水传染的微生物。对其低浓度的检测方法有去孢子计数等。在本研究中，为了开发更方便的系统，采用细胞培养作为载体组织。该细胞由人小肠癌细胞HCT-8培养而成。ELISA法检测隐孢子虫可感染。作为对有机体消毒的目的，我们将其感染潜力集中在细胞上。活细胞和固定细胞培养表现出感染细胞与正常细胞的差异。人们发现紫外线能有效地降低这种有机体的传染潜力。紫外线强度为20毫瓦秒/厘米^2。