Synthesis and Extended Functions of Intelligent Large Sugar Balls

本研究涉及基于肌氨酸N-羧酸酐（NCA）的径向生长聚合（RGP）的介观大分子结构，即，N-甲基甘氨酸NCA与聚（三亚甲基亚胺）树枝状聚合物和与聚（酰氨基胺）树枝状聚合物反应以产生线性聚合物-超连接的树枝状聚合物。值得注意的是，星形嵌段共聚物通过与树枝状引发剂的一锅反应方便地制备。在该聚合体系中，传播载体具有由作为引发剂核的树枝状聚合物的末端活性端引发的径向对称性的顺序。由于采用了快速引发/缓慢增长体系，线性段的数量严格取决于树枝状聚合物的生成。因此，本发明的多重聚合体系不同于用于制备星星聚合物的常规聚合。除了合成方面，还利用小角中子散射（SANS）研究了星形树枝状大分子的大小和形状，并利用该树枝状大分子引发糖取代氨基酸N-羧基氰脲酸（glycoNCAs）的活性聚合，制备了智能大糖球。用小麦胚芽凝集素（WGA）进行红细胞凝集抑制试验，检测大树枝状分子的分子识别能力。通过电泳进一步研究了星星树状大分子的复合物形成能力。

The present study is concerned with mesoscopic macromolecular architecture based on radial-growth polymerization (RGP) of sarcosine N-carboxyanhydride (NCA), i.e., N-methylglycine NCA, with poly(trimethyleneimine) dendrimer and with poly(amido amine) dendrimer to generate a linear polymer-hyperlinked dendrimer. It is noteworthy that the star-shaped block copolymer is conveniently prepared by one-pot reaction with the dendritic initiator. In this polymerization system, propagating vectors have an order of a radial symmetry initiated by terminal active ends of the dendrimer as an initiator core. Numbers of the linear segment are strictly determined by generation of the dendrimer, since the fast initiation/slow propagation system is adopted. Thus, the present multiple polymerization system is different from conventional polymerizations employed for preparation of star polymers. Besides the synthetic aspect, size and shape of the star-shaped dendrimer were investigated by small-angle neutron scattering (SANS).Living polymerization of sugar-substituted amino acid N-carboxyanhydrides (glycoNCAs) initiated with the dendrimers was also undertaken to afford intelligent large sugar balls. Molecular recognition abilities of the large dendrimer were examined by erythrocyte agglutination inhibition assays using wheet germ agglutinin (WGA). Complex formation ability of the star dendrimers was further investigated by electrophoresis.