Studies on bulb formation and dormancy in bulbous plants by molecular biological approach

利用分子生物学方法对鳞茎形成的诱导和鳞茎休眠的诱导是同一现象的假说进行了研究。以风信子（Hyacinthus orientalis L.）CV.德尔夫特蓝），在MS + NAA + BA培养基上培养。5℃低温处理4个月后，外植体形成鳞茎，而未经低温处理的外植体形成鳞茎受到抑制。阿坝促进鳞茎的形成，而不冷处理。flundone抑制了冷处理外植体的鳞茎形成。在鳞茎形成条件下，内源阿坝含量增加。这些结果表明，鳞茎的形成受低温诱导，并受阿坝的调控。因此，控制方法是通过冷处理或不经冷处理的BAB可获得鳞茎形成条件，并且非鳞茎形成条件是通过非冷处理或氟啶酮与冷处理。 关于我们 研究了低温和阿坝对风信子鳞茎形成的影响。冷处理和非冷处理的拍摄样品之间的差异PCR扩增片段进行了观察。6个差异PCR扩增片段在冷处理和ABA处理中共同表达，2个差异PCR扩增片段在冷处理和ABA处理中被抑制。对低温处理后得到的18个特异性基因片段进行了部分序列测定（命名为HL克隆）。经FASTA程序鉴定，8个HL克隆与已鉴定序列的同源性在80%以上。低温和阿坝诱导的HL cDNA克隆与A. thaliana或O. sativa. 3个HL克隆与I.红薯一些HL克隆与功能已被提出的基因或mRNA同源。功能尚不明确的HL克隆可能是与鳞茎形成相关的新基因。少

Molecular biological studies were used to investigate the hypothesis that the induction of bulb formation and the induction of bulb dormancy are the same phenomenon. Shoot explants were taken from in vitro immature leaves of hyacinth (Hyacinthus orientalis L. cv. Delft Blue) and cultured at 25℃ on MS medium containing NAA and BA. The explants formed bulbs four after the cold treatment at 5℃, whereas bulb formation was supressed without cold treatment. ABA promoted bulb formation without cold treatment. By flundone, bulb formation of the cold treated explants was inhibited. Under bulb-forming conditions, endogenous ABA content increased. These results suggest that bulb formation is induced by low temperature and regulated by ABA. Thus, the controlling methods are bulb forming conditions are obtainable by cold treatment or BAB without cold treatment and non-bulb forming conditions are by non-cold treatment or fluridone with cold treatment.Differential display analysis of genes induced by … More low temperature and ABA in bulb formation of hyacinth was conducted. Differential PCR amplified fragments between cold-treated and non-cold-treated shoot samples were observed. Six differential PCR amplified fragments were commonly expressed both in the cold and ABA treatments, and two were suppressed in both treatments. The cDNA amplified fragments observed in both treatments might be related to bulb formation in hyacinth.The partial sequences of the cDNA clones obtained from 18 specific gene fragments by low temperature (designated as HL clones) were carried out. Eight HL clones had over 80% identity with identified sequences with FASTA program. The HL clones of cDNA induced by low temperature and ABA were homologous to genome DNA of A. thaliana or O. sativa. Three HL clones showed partial identity with starch phosphorylase gene of I. batatas. Some HL clones were homologous to genes or mRNAs of which the function had been suggested. It is possible that HL clones of which the functions were not clarified are novel genes related to bulb formation. Less