The Relationship between genetic distance, environment and the properties on Sugi

杉树遗传距离、环境与性状的关系

• 批准号: 12460073
• 负责人: NAKAMURA Noboru
• 金额: $ 2.94万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460073/
• 关键词: genetic distance; environment; properties; Sugi

In order to analyze the relation between relationship between genetic distance, environment and the properties on Sugi, the following three subjects were investigated.(1) Measurement of the properties on young Sugi seedlings Young's moduli(MOE) of young Sugi seedlings were measured. The results are followed :【○!1】 MOE of young Sugi seedlings could be measured as previous studies.【○!2】 The correlation between MOE and height or diameter of young Sugi seedlings, the indexes of growth, was not recognized.【○!3】 We can select the seedlings which have relatively high MOE and the capability of relatively rapid growth.【○!4】 MOE becomes larger as the measured position of a seedling become higher.From these results, it is expected to breed Sugi with high MOE by selecting young seedlings with high MOE and make maps of Sugi Young's MOE from the viewpoint of using Sugi as structural components and end use.(2) Seting the experimental locationsThe six experimental locations were set up in the university forests of Kyushu University, Ehime University, Mie University, Shizuoka University, Niigata University and Hokkaido University.(3) Analysis of genetic distance of Sugi breeds by RAPD methodMany joint DNA markers have been developed by the sequence information gotten from the Sugi cDNA libraries. Using these markers, DNA of 25 Sugi families, collected from the whole country, were analyzed. These 25 families were classified to 2 groups, the Nippon Sea group and the Pacific Sea one. It is suggested that Ura-sugi genealogy and Omote-sugi one, which were classified from figural features, are main two genealogies from the viewpoint of Genom. Okinoyamasugi, Tateyamasugi and Sadogashima used in this research are included in Ura-sugi genealogy, on the other hand Sanbusugi is in Omote-sugi genealogy. There is strong possibility that these genealogies are distance off genetically.

为了分析遗传距离、环境与杉属植物性状之间的关系，对以下三个课题进行了研究。(1)对杉幼苗的性质的测量测量了杉幼苗的杨氏模量（莫伊）。结果如下：[○！1]幼苗的莫伊可以像以前的研究一样测量。○！2]莫伊与苗木高、地径等生长指标之间的相关性不明显。○！3]可以选择莫伊相对较高、生长能力相对较快的幼苗。○！4]从这些结果来看，可以期待通过选择MoE高的幼苗来培育MoE高的杉树，并从将杉树作为结构成分和最终用途的观点出发，制作杉树的MoE地图。(2)设置实验地点在九州大学、爱媛大学、三重大学、静冈大学、新泻大学和北海道大学的大学森林中设置了6个实验地点。(3)利用RAPD技术分析杉属植物间的遗传距离利用杉属植物cDNA文库中的序列信息，已经开发出许多联合DNA标记。利用这些标记对采自全国的25个杉科家系的DNA进行了分析。这25个科被分为两个组，日本海组和太平洋组。从基因组学的观点来看，浦杉系谱和表针杉系谱是主要的两个系谱，它们是从形态特征来划分的。本研究中使用的冲之山杉、立山杉和佐渡岛杉属于浦杉谱系，而三不杉属于表本杉谱系。有很大的可能性，这些家谱是距离遗传。