ANALYSIS OF IMMUNE REGULATION BY ADHESION MOLECULE COMPLEX ON KILLER LYMPHOCYTES

粘附分子复合物对杀伤淋巴细胞免疫调节的分析

• 批准号: 12470111
• 负责人: SHIBUYA Akira
• 金额: $ 6.08万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470111/
• 关键词: DNAM-1; killer lymphocytes; viral infection; cancer; CD155; CD1112

Cytotoxic lymphocytes play an important role for immune defense against virus-infected cells and cancer. Intercellular adhesion molecule is involved in binding between killer lymphocytes and target cells. We previously identified a novel adhesion molecule DNAM-1 (CD226). Here we have explored the regulatory mechanisms of intercellular binding and signaling mediated by DNAM-1, leading to fundamental understanding for immune defense against virus infection and cancer. Moreover, we have also identified CD155 and CD112 as the DNAM-1 ligands. DNAM-1 binding with its ligands mediates activation signal in cytotoxic Lymphocytes. In addition, we have demonstrated that DNAM-1 also plays an important role for regulation of immune system and thrombosis.

细胞毒性淋巴细胞在免疫防御病毒感染细胞和癌症中起重要作用。细胞间粘附分子参与杀伤淋巴细胞和靶细胞之间的结合。我们先前鉴定出一种新型的粘附分子DNAM-1（CD226）。在这里，我们探索了DNAM-1介导的细胞间结合和信号传导的调节机制，从而对针对病毒感染和癌症进行免疫防御的基本了解。此外，我们还确定CD155和CD112为DNAM-1配体。 DNAM-1与其配体的结合介导细胞毒性淋巴细胞中的激活信号。此外，我们已经证明DNAM-1在调节免疫系统和血栓形成方面也起着重要作用。

项目成果

Sakamoto N., et al.: "A novel Fc receptor for lgA and lgM is expressed on both hematopoietic and non-hematopoietic tissues"Eur J Immunol. 31. 1310-1316 (2001)

Sakamoto N. 等人：“IgA 和 IgM 的新型 Fc 受体在造血组织和非造血组织上均表达”Eur J Nutrition。

Tokoro Y, et al.: "Molecular cloning and characterization of mouse Tspan-3, a novel member of the tetraspanin superfamily"Biochem.Biophys.Res.Commu.. 288. 178-183 (2001)

Tokoro Y 等人：“小鼠 Tspan-3（四跨膜蛋白超家族的新成员）的分子克隆和表征”Biochem.Biophys.Res.Commu.. 288. 178-183 (2001)

Shimizu Y, et al.: "Fc/mu R is a single gene-family member closely related to polymeric Ig receptor on chromosome 1"Immunogenetics. 42. 709-711 (2001)

Shimizu Y 等人：“Fc/mu R 是与 1 号染色体上的聚合 Ig 受体密切相关的单基因家族成员”免疫遗传学。

Iwama A., et al.: "Reciprocal roles for C/EBP and pu.l transcription factors in hangerhans cell commitment"J.Exp.Med. 195. 547-558 (2002)

Iwama A. 等人：“C/EBP 和 pu.l 转录因子在hangerhans 细胞定型中的相互作用”J.Exp.Med。

Tahara-Hanaoka S., Shibuya K., et al., Shibuya A.: "Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD 155) and Nectin-2 (PRR2/CDll2)."Int Immunol. (in press).

Tahara-Hanaoka S.、Shibuya K. 等人、Shibuya A.：“DNAM-1 (CD226) 与其配体 PVR (CD 155) 和 Nectin-2 (PRR2/CDll2) 相互作用的功能表征。”Int 免疫学