ANALYSIS OF IMMUNE REGULATION BY ADHESION MOLECULE COMPLEX ON KILLER LYMPHOCYTES

粘附分子复合物对杀伤淋巴细胞免疫调节的分析

• 批准号: 12470111
• 负责人: SHIBUYA Akira
• 金额: $ 6.08万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470111/
• 关键词: DNAM-1; killer lymphocytes; viral infection; cancer; CD155; CD1112

Cytotoxic lymphocytes play an important role for immune defense against virus-infected cells and cancer. Intercellular adhesion molecule is involved in binding between killer lymphocytes and target cells. We previously identified a novel adhesion molecule DNAM-1 (CD226). Here we have explored the regulatory mechanisms of intercellular binding and signaling mediated by DNAM-1, leading to fundamental understanding for immune defense against virus infection and cancer. Moreover, we have also identified CD155 and CD112 as the DNAM-1 ligands. DNAM-1 binding with its ligands mediates activation signal in cytotoxic Lymphocytes. In addition, we have demonstrated that DNAM-1 also plays an important role for regulation of immune system and thrombosis.

细胞毒性淋巴细胞在抵抗病毒感染细胞和癌症的免疫防御中发挥重要作用。细胞间粘附分子参与杀伤淋巴细胞与靶细胞的结合。我们先前鉴定了一种新的粘附分子DNAM-1（CD 226）。在这里，我们探索了由DNAM-1介导的细胞间结合和信号传导的调节机制，从而对病毒感染和癌症的免疫防御有了基本的了解。此外，我们还鉴定了CD 155和CD 112为DNAM-1配体。DNAM-1与其配体结合介导细胞毒性淋巴细胞中的活化信号。此外，我们已经证明DNAM-1在免疫系统和血栓形成的调节中也起着重要作用。

项目成果

Tokoro Y, et al.: "Molecular cloning and characterization of mouse Tspan-3, a novel member of the tetraspanin superfamily"Biochem.Biophys.Res.Commu.. 288. 178-183 (2001)

Tokoro Y 等人：“小鼠 Tspan-3（四跨膜蛋白超家族的新成员）的分子克隆和表征”Biochem.Biophys.Res.Commu.. 288. 178-183 (2001)

Sakamoto N., et al.: "A novel Fc receptor for lgA and lgM is expressed on both hematopoietic and non-hematopoietic tissues"Eur J Immunol. 31. 1310-1316 (2001)

Sakamoto N. 等人：“IgA 和 IgM 的新型 Fc 受体在造血组织和非造血组织上均表达”Eur J Nutrition。

Shimizu Y, et al.: "Fc/mu R is a single gene-family member closely related to polymeric Ig receptor on chromosome 1"Immunogenetics. 42. 709-711 (2001)

Shimizu Y 等人：“Fc/mu R 是与 1 号染色体上的聚合 Ig 受体密切相关的单基因家族成员”免疫遗传学。

Motohashi T, et al.: "Increased cell surface expression of C-terminal truncated erythropoietin receptors in polycythemia"Eur J Haematol. 67. 88-93 (2001)

Motohashi T 等人：“红细胞增多症中 C 末端截短的促红细胞生成素受体的细胞表面表达增加”Eur J Haematol。

Tahara-Hanaoka S., Shibuya K., et al., Shibuya A.: "Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD 155) and Nectin-2 (PRR2/CDll2)."Int Immunol. (in press).

Tahara-Hanaoka S.、Shibuya K. 等人、Shibuya A.：“DNAM-1 (CD226) 与其配体 PVR (CD 155) 和 Nectin-2 (PRR2/CDll2) 相互作用的功能表征。”Int 免疫学