Gene expression profiling of biliary epithelial cells in primary biliary cirrhosis : laser microdissection and microarray analysis

原发性胆汁性肝硬化胆管上皮细胞的基因表达谱：激光显微切割和微阵列分析

• 批准号: 12470122
• 负责人: YAMAMOTO Kazuhide
• 金额: $ 9.28万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470122/
• 关键词: Primary biliary cirrhosis; Laser microdissection; Biliary epithelial cells; DNA microarray; DNA マイクロアレイ

Although primary biliary cirrhosis (PBC) is an organ-specific autoimmune disease, target molecules remains unidentified. We attempted to identify target molecules in biliary epithelial cells of PBC by laser capture microdissection (LCM) and micro-array technology.Preliminary experiments showed successful collection of pure biliary epithelial cells and amplification of messages by a T7 RNA polymerase based method.Gene expression profiles of biliary epithelial cells were compared among normal, chronic hepatitis C and PBC patients. Biliary epithelial cells from PBC patients demonstrated increased expression of some genes compared with normal biliary cells.However, some of the genes showing increased expression were also increased in biliary epithelial cells from chronic hepatitis C patients, suggesting that the increased expression might be induced by portal inflammation. Analysis of PBC specific gene expressions is now under study.

虽然原发性胆汁性肝硬化（PBC）是一种器官特异性自身免疫性疾病，但靶分子仍不清楚。本研究采用激光捕获显微切割（LCM）技术和微阵列技术，成功采集了PBC患者胆管上皮细胞，并利用T7 RNA聚合酶扩增了胆管上皮细胞中的靶分子，比较了正常人、慢性丙型肝炎患者和PBC患者胆管上皮细胞的基因表达谱。PBC患者的胆管上皮细胞中某些基因的表达较正常胆管上皮细胞高，而慢性丙型肝炎患者的胆管上皮细胞中某些基因的表达也较正常胆管上皮细胞高，这可能是门静脉炎症所致。PBC特异性基因表达的分析目前正在研究中。

项目成果

Terada R, et al.: "Stromal cell derived factor-1 from biliary epithelial cells recruits CXCR4-positive cells"Lab Invest. (In press).

Terada R 等人：“来自胆管上皮细胞的基质细胞衍生因子 1 招募 CXCR4 阳性细胞”Lab Invest。

Terada R, et al.: "Stromal cell-derived factor-1 from biliary epithelial cells recruits CXCR4-positive cells : implications for inflammatory liver diseases"Lab Invest. (in press).

Terada R 等人：“来自胆管上皮细胞的基质细胞衍生因子 1 招募 CXCR4 阳性细胞：对炎症性肝病的影响”Lab Invest。

Yamamoto K, et al.: "A scoring system for primary biliary cirrhosis and its application for variant forms of autoimmune liver disease"J Gastroenterol. 38. 52-59 (2003)

Yamamoto K 等人：“原发性胆汁性肝硬化的评分系统及其在自身免疫性肝病变异形式中的应用”J Gastroenterol。

Yamamoto K, et al.: "A scoring system for primary biliary cirrhosis and its application for variant forms of autoimmune liver disease"J Gastroenterol. 38(1). 52-59 (2003)

Yamamoto K 等人：“原发性胆汁性肝硬化的评分系统及其在自身免疫性肝病变异形式中的应用”J Gastroenterol。

Matsumura S, et al.: "Comprehensive mapping of HLA-A0201-restricted CD8 T-cell epitopes"Hepatology. 36. 1125-1134 (2002)

Matsumura S 等人：“HLA-A0201 限制性 CD8 T 细胞表位的综合作图”肝病学。