laser microdissection microscope

激光显微切割显微镜

• 批准号: 503250411
• 金额: --
• 依托单位: Universität zu Köln
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/503250411?language=en
• 关键词: laser; microdissection; microscope

The microscope and accessories requested are intended for use in the Proteomics, Transcriptomics, and Imaging Core Facilities at CECAD. Recent developments in next-generation sequencing (NGS) and mass spectrometry have enabled the unbiased analysis of samples from small amounts of tissue, single cells, and even isolated nuclei. To isolate specific cell populations, individual cells and their subcompartments in an automated, high-throughput manner, modern laser microdissection (LMD) systems have been combined with deep-learning and machine learning-based imaging analysis tools. The acquisition of a modern LMD microscope will therefore be a vital asset to the projects carried out by the research groups at the Cologne campus. Furthermore, due to the high number of samples required for proteomics and transcriptomics, it is important to integrate an automated and efficient cell classification system with high-resolution imaging. Platforms for NGS and sensitive mass spectrometers are well-established in Cologne and enable analysis of complex biological samples. Since the demand for quantitative analyses of individual cell populations has massively increased, a new LMD microscope will be essential to the realization of the planned research projects.

所要求的显微镜和附件预期用于CECAD的蛋白质组学、转录组学和成像核心设施。下一代测序（NGS）和质谱的最新发展使得能够对来自少量组织、单细胞甚至分离的细胞核的样品进行无偏分析。为了以自动化、高通量的方式分离特定的细胞群、单个细胞及其亚区室，现代激光显微切割（LMD）系统已与基于深度学习和机器学习的成像分析工具相结合。因此，购买一台现代化的LMD显微镜将成为科隆校区研究小组开展项目的重要资产。此外，由于蛋白质组学和转录组学所需的大量样品，将自动化和高效的细胞分类系统与高分辨率成像相结合是很重要的。科隆的NGS平台和灵敏的质谱仪已经建立，可以分析复杂的生物样品。由于对单个细胞群定量分析的需求大幅增加，新的LMD显微镜对于实现计划的研究项目至关重要。