Adaptation capacity of small intestine in short bowel syndrome and criteria for small intestinal transplantation

短肠综合征小肠适应能力及小肠移植标准

基本信息

批准号：
12470373
负责人：
SUITA Sachiyo
金额：
$ 9.41万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470373/
关键词：
piglet short bowel syndrome tissue blood flow of liver portal blood flow liver dysfunction liver fibrosis small intestinal transplantation massive resection of small bowel 短腸症候群モデル小腸大量切徐新生児門脈血流計空腸回腸

项目摘要

Background : Regarding complications associated with short bowel syndrome(SBS), progressive liver failure is one of most severe complications known to occur. Although several studies have suggested that many factors interactively influence on this clinical condition, we investigated the relationship between hepatic circulation and hepatic fibrosis, using a neonatal piglet SBS model.Materials & Methods : This study used the following four groups of neonatal piglets : a group with an 80% resection of the small bowel(SBS group), a group with a by-pass operation of the small bowel(functional SBS group), a group with only a laparotomy as a Sham operation(Sham group), and a no operative treatment group(Control group). We measured the hepatic circulation just before and after the reconstruction of the intestine, as well as on 7th and 14th postoperative day. In addition, both blood and hepatic tissue samples were collected to investigate them both biochemically and morphologicallyResults : In the biochemical liver function and the tissue blood flow of liver, there were no significant differences among all groups on any investigated days. However, on both the 7th and 14th postoperative day, the portal venous flow in the SBS group was significantly lower than that in other groups. According to a histological analysis, only hepatic samples on the 14th postoperative day showed mild hepatic fibrosis in the SBS group. In □-smooth muscle actin staining, which expresses active stellate cells, numerous positive cells were distributed in the perisinusoidal space on the 14th postoperative day in the SBS group.Conclusion : Based on our data, a decrease in the hepatic circulation, especially in the portal venous flow, after a massive resection of the intestine, may cause progressive liver dysfunction due to the activation of hepatic stellate cells.

背景：关于与短肠综合征（SBS）相关的并发症，进行性肝衰竭是已知发生的最严重并发症之一。 Although several studies have suggested that many factors interactively influence on this clinical condition, we investigated the relationship between hepatitic circulation and hepatitic fibrosis, using a neonatal piglet SBS model.Materials & Methods: This study used the following four groups of neonatal piglets: a group with an 80% resection of the small bowel(SBS group), a group with a by-pass operation of the small bowel(functional SBS group), a group with仅作为假手术（假手术组）和无手术治疗组（对照组）。我们在肠重建之前和之后测量了肝循环，以及术后第7和14日。此外，收集了血液和肝组织样品，以在生化和形态学上研究它们：在生化肝功能和肝脏的组织血流中，在任何研究的日子中，所有组之间均无显着差异。但是，在术后第7和14日，SBS组的门静脉流量明显低于其他组的静脉流量。根据组织学分析，仅在术后第14天，只有肝样品显示SBS组的轻度肝纤维化。在□ - 表达活跃的星状细胞的光滑肌肉肌动蛋白染色中，在SBS组的第14天，在术后第14天分布了许多阳性细胞。结论：基于我们的数据，肝循环的减少，尤其是在门静脉流动，导致渐进式渐进性，可能会导致渐进性，可能会导致渐进式渐进性，可能会导致渐进式渐进性。星状细胞。