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Effect of growth factor regarding lung development of congenital diaphragmatic hernia

生长因子对先天性膈疝肺发育的影响

基本信息

批准号：
16390504
负责人：
SUITA Sachiyo
金额：
$ 9.28万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390504/
关键词：
Congenital diaphragmatic hernia Lung development Insulin like growth factor Organ culture Fetal treatment Gene induction Growth hormone Immunohistochemistry 経胎盤的胎児麻酔サイトカインサーファクタント

项目摘要

Insulin-like growth factors (IGFs) are one of the major growth factors related to lung development. The purpose of this study is to analyze the effect of IGF using organ cultured lungs.The lungs were dissected from E11 to E18 mice. Total RNA was then extracted from those lungs. The expression of IGF and its receptors was analyzed by RT-PCR.The lungs dissected from E17 mice were cultured in the BGjb medium for 48 hours. The lungs were divided into the following three groups ; the lungs were cultured only by the medium (group I), or the medium containing either IGF-1 (group II) or IGF-2 (group III). The expressions of TTF-1,Sp-C, and alpha-SMA were analyzed by both RT-PCR and immunohistochemistry.The m-RNA expression of IGF-1 receptor was observed to be higher from E16 to E18 than at any other embryonic stages. TTF-1,Sp-C and alpha-SMA were more highly expressed in groups II and III than in those of group I. TTF-1,Sp-C, and alpha-SMA positive cells were increased in group II and III compared to group I.Based on our findings, IGF is therefore suggested to induce alveolar maturation in the late stages of fetal lung development. IGF is considered to be one of the candidates for promoting lung development in patients with congenital diaphragmatic hernia.

胰岛素样生长因子（IGF）是与肺部发育相关的主要生长因子之一。本研究的目的是利用器官培养的肺来分析 IGF 的作用。解剖 E11 至 E18 小鼠的肺。然后从这些肺部提取总RNA。 RT-PCR分析IGF及其受体的表达。将E17小鼠的肺解剖在BGjb培养基中培养48小时。肺分为以下三组：仅用培养基（I组）或含有IGF-1（II组）或IGF-2（III组）的培养基培养肺。采用RT-PCR和免疫组织化学方法分析TTF-1、Sp-C和α-SMA的表达。观察到E16至E18期间IGF-1受体的mRNA表达高于任何其他胚胎阶段。 TTF-1、Sp-C和α-SMA在II组和III组中的表达高于I组。与I组相比，II组和III组中TTF-1、Sp-C和α-SMA阳性细胞增多。因此，根据我们的研究结果，表明IGF在胎肺发育后期诱导肺泡成熟。 IGF被认为是促进先天性膈疝患者肺部发育的候选药物之一。