The Evaluetion of The Hypersensitivity Depression Effect Using The Hypersensitive Dentine Simulator

使用过敏性牙本质模拟器评估过敏性抑郁效应

• 批准号: 12470402
• 负责人: ISHIHATA Hiroshi
• 金额: $ 4.42万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470402/
• 关键词: HYPERSENSITIVE; SIMULATOR; LUMINESCENCE; PHOTODIODE; LUMINOL; DENTINE; シミュンータ; 抵抗値; LED

Dentine perception is evoked by the transfer of internal fluid in the dentine microtubule according to the hydrodynamics theory, therefore, the technique which measures the deterrence of the transfer of dentinal tubule internal fluid seems to be useful in order to objectively evaluate the hypersensitive dentine control material. Ten extracted human third molar teeth were used in this study. The dentine slices at the crown part of 1 and 1.5mm thickness were prepared in tooth axis vertical surface. These slices were fixed in the 10% neutral formalin solution. The dentine microtubule were opened to wash in the experiment by 3O% hydrogen peroxide water. From both slice sides where the dentine microtubule in the tooth crown and tooth pulp side opened, these dentine slices were held with the O-ring by the transparent acrylic tube. 1% sodium hydroxide solution in the acrylic tube in the tooth pulp side with the 0.02% luminol was filled. 1% potassium ferricyanide solution including the 0.3% hy … More drogen peroxide water which similarly removed the fine particle in the acrylic tube in the tooth crown side by the Millipore Corp. filter of ostia size of 0.2 μm was satisfied. The photodiode (s6204 : Hamamatsu Photonics Co.)which contained preamplifier and thermoelectric cooling device by having approximately 30mm distances in the neighborhood of the sample was installed. The equipment with sample was installed in the black box which almost perfectly cuts off the outside light. The light emission of luminol chemiluminescence evoked by the active oxygen of hydrogen peroxide water is generated by the mixture of the reagents filled in the acrylic tube of both sides through dentine microtubules. The luminous phenomenon was detected in the photodiode. The optical output from the photodiode was recorded through the AD converter in personal computer after observing by the oscilloscope. In the condition that the reagent just contacted both dentine slice sides, there was a slight light emission of the transience. The light emission was observed time-dependent, when 10〜500mmHg air pressure was applied for the acrylic tube that the potassium ferricyanide and hydrogen peroxide solution was filled. These light emission quantity had the correlation which was positive with the pressure in the same sample, though there is the fluctuation in proportion to individual sample, and the light emission quantity changed. At the light emission quantity in using slice samples of the 1.0mm thickness which was excellent in the permeability in the dentine, the light emission quantity tended to generally increase in comparison with 1.5mm samples. In the condition without opening of dentine microtubule by 30% hydrogen peroxide water, the dentine sample could not demonstrate the light emission even in any loading of reagent. It is a system that dentine hypersentive simulator using the chemiluminescence visualizes the transfer of the internal fluid in dentine microtubule and that it can be supersensitized, and that it can be observed. It is possible to show the degree of the opening of the dentine microtubule in fluctuation situation of the light emission quantity. This system seems to be very much the usefulness as the tool which can straightforwardly evaluate the effect of the control material for dentine hypersensitive disease. Less

根据流体力学原理，牙本质感知是由牙本质微管内液体的转移引起的，因此，测量牙本质小管内液体转移的阻止性的技术似乎是有用的，以客观地评价过敏性牙本质控制材料。在这项研究中使用了10个提取的人类第三磨牙。在牙轴垂直面制备1和1.5mm厚的牙冠部牙本质切片。将这些切片固定在10%中性福尔马林溶液中。实验中用30%过氧化氢水冲洗牙本质微管。从牙冠和牙髓侧的牙本质微管开口的两个切片侧，通过透明丙烯酸管用O形环保持这些牙本质切片。将1%的氢氧化钠溶液在丙烯酸管中的牙髓侧填充有0.02%的鲁米诺。1%铁氰化钾溶液，包括0.3%HY ...更多信息 过氧化氢水，其类似地通过孔尺寸为0.2 μm的Millipore Corp.过滤器去除牙冠侧的丙烯酸管中的细颗粒，是令人满意的。光电二极管（s6204：Hamamatsu Photonics Co.）其通过在样品附近具有大约30 mm的距离安装包含热电和热电冷却装置。带样品的设备安装在黑匣子中，几乎完美地切断了外部光线。过氧化氢水的活性氧诱发的鲁米诺化学发光的光发射是由填充在两侧丙烯酸管中的试剂的混合物通过牙本质微管产生的。在光电二极管中检测到发光现象。通过示波器观察后，通过个人计算机中的AD转换器记录光电二极管的光输出。在试剂与牙本质切片两侧接触的情况下，有轻微的瞬态发光。当对装有铁氰化钾和过氧化氢溶液的丙烯酸管施加10 ~ 500 mmHg的气压时，观察到光发射随时间而变化。这些发光量在同一样品中与压力呈正相关，但个别样品存在成比例的波动，发光量发生变化。在使用在牙本质中的渗透性优异的1.0mm厚度的切片样品中的光发射量时，与1.5mm样品相比，光发射量通常倾向于增加。在30%过氧化氢水不开放牙本质微管的条件下，牙本质样品在任何试剂加载量下都不能显示光发射。它是一种利用化学发光的牙本质高敏模拟器可视化牙本质微管内液体的转移并可超敏且可观察的系统。在发光量的波动情况下，可以显示牙本质微管的开放程度。该系统似乎非常有用，作为工具，可以直接评估的控制材料的效果，牙本质过敏症。少