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Relationship among anabolic androgenic steroid, weight-lifting exercise and physiological functions.

合成代谢雄激素类固醇、举重运动与生理功能之间的关系。

基本信息

批准号：
12480012
负责人：
TAMAKI Tetsuro
金额：
$ 9.22万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The influence of an anabolic androgenic steroid (AAS) on thymidine and amino acid uptake in rat hindlimb skeletal muscles after a single exhaustive bout of weight-lifting was determined. Nandrolone decanoate or oil vehicle was administered to the Steroid group one week prior to the experiments. Serum creatine kinase (CK) activity, used as a measure of muscle damage, increased 30 and 60 min after exercise in both groups. The total amount of weight lifted was higher, whereas CK levels were lower in Steroid than Control rats. Thymidine uptake (cellular mitotic activity) peaked two days after exercise in both groups and was 90% higher in Control than Steroid rats, reflecting a higher level of muscle damage. Leucine uptake (amino-acid metabolism) was ~80% higher at rest and recovered 33% faster post-exercise in Steroid than Control rats. The in situ isometric mechanical properties of the plantaris muscle were determined. The only significant difference was a higher fatigue resistance in the … More Steroid compared to the Control group. These results indicate that AAS treatment 1) ameliorates CK efflux and muscle fiber damages and enhances the rate of protein synthesis and 2) enhances in vivo work capacity and the in situ fatigue resistance of a primary plantarflexor muscle. To identify possible mechanisms for AAS induced increase in aggressive behavior and work capacity, the levels of some biogenic amines were measured in selected regions of the brain. The levels of norepinephrine and its metabolite MHPG (4-hydroxy-3- methoxyphenylglycol) were increased by -2- and -7-fold in the hypothalamus of the Steroid compared to the Control and Vehicle groups. The levels of serotonin and its metabolite 5-HIAA (5-hydroxy-indole-3-acetic acid) were -40 and -50% higher in the Steroid compared to the Control and Vehicle groups. A significantly higher number of c-fos expressing neurons were observed in the periventricular region of the Steroid than the Control and Vehicle groups, indicating enhanced neuronal activity following nandrolone decanoate treatment Combined, the physical performance enhancement following anabolic-androgenic steroid treatment, are consistent with the interpretation that elevated levels of adrenergic and serotonergic amines in the hypothalamus could contribute to aggressive behaviors as well as improved physical performance. Less

确定了单次力竭举重后，合成代谢雄激素类固醇（AAS）对大鼠后肢骨骼肌胸苷和氨基酸摄取的影响。实验前一周向类固醇组施用癸酸诺龙或油载体。两组的血清肌酸激酶 (CK) 活性（用于衡量肌肉损伤）在运动后 30 分钟和 60 分钟均有所增加。与对照大鼠相比，类固醇大鼠的举重总量较高，而 CK 水平较低。两组的胸苷摄取（细胞有丝分裂活性）在运动后两天达到峰值，并且对照组比类固醇大鼠高 90%，反映了更高水平的肌肉损伤。与对照大鼠相比，类固醇大鼠在休息时亮氨酸摄取（氨基酸代谢）高出约 80%，运动后恢复速度快 33%。确定了跖肌的原位等距力学特性。唯一显着的差异是类固醇组与对照组相比具有更高的抗疲劳性。这些结果表明，AAS 治疗 1) 改善 CK 流出和肌纤维损伤并提高蛋白质合成速率，2) 增强初级跖屈肌的体内工作能力和原位疲劳抵抗力。为了确定 AAS 诱导攻击行为和工作能力增加的可能机制，在大脑的选定区域测量了一些生物胺的水平。与对照组和赋形剂组相比，类固醇组下丘脑中去甲肾上腺素及其代谢物 MHPG（4-羟基-3-甲氧基苯基乙二醇）的水平增加了 -2 倍和 -7 倍。与对照组和赋形剂组相比，类固醇组中的血清素及其代谢物 5-HIAA（5-羟基吲哚-3-乙酸）水平分别高出 -40% 和 -50%。在类固醇的心室周围区域观察到表达 c-fos 的神经元数量显着高于对照组和赋形剂组，表明癸酸诺龙治疗后神经元活性增强。综合而言，合成代谢雄激素类固醇治疗后的身体表现增强，与下丘脑中肾上腺素能和血清素能胺水平升高可能导致攻击行为的解释一致，如下：以及改善的身体表现。较少的