New method for the analysis of motor function on experimental animals.

分析实验动物运动功能的新方法。

• 批准号: 07680142
• 负责人: TAMAKI Tetsuro
• 金额: $ 1.41万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680142/
• 关键词: skeletal muscle; motor system; physiological function; in-vivo; computer analysis; in-situ

Newly modified method that could be quantitative the motor function on laboratory animals was established. This method is based on the computer analysis for electrically evoked muscle contractions and EMG.Contractile properties and EMG of the compensatory hypertrophied rat model and senile (over 2 year-old) rats were measured under urethane anesthetized condition, and compared with those of control rats. Twitch and tetanus of compensatory hypertrophied muscle (CHM) were slower than control muscle, and this tendency was supported by the histochemical analysis. However, the fatigue resistance capacity of CHM was illogically less than that of control muscles. At the same time, I found that CHM was associated with the appearance of numerous complex branched fibers (CBF). I also confirmed that CBF was also observed in the muscles of muscular dystrophy mouse (mdx : Tamaki et al., Anat.Rec.1993) and in the muscles repeatedly altered by bupivacaine hydrochloride (Tamaki and Akatsuka, Anat.Rec.1994). Thus, possibility is suggest that the presence of CBF may disturb the correlation between histochemical and functional properties of the whole muscle. In the senile rats, properties of twitch and tetanus were also slower than control rats. In the control rats, complete tetanus could be always obtained at 100Hz of stimulation frequency. However, some of senile rats could not follow over 50Hz stimulation. It is suggest that this is very important and interesting fact to consider the effects of aging for motor function. In conclusion, this "Newly modified method for the analysis of motor function on laboratory animals" is useful to quantitative the motor function of various laboratory animals.

建立了一种新的实验动物运动功能定量方法。该方法是基于电诱发肌肉收缩和肌电图的计算机分析。测量代偿性肥厚模型大鼠和老年（2岁以上）大鼠在氨基甲酸乙酯麻醉下的收缩特性和肌电图，并与对照大鼠进行比较。代偿性肥厚肌（CHM）的抽搐和破伤风比对照肌慢，这一趋势得到组织化学分析的支持。然而，中轴肌的抗疲劳能力明显低于对照肌。同时，我发现CHM与大量复杂分枝纤维（CBF）的出现有关。我还证实，在肌营养不良小鼠的肌肉中也观察到CBF （mdx: Tamaki et al., Anat.Rec）。1993)和反复被盐酸布比卡因改变的肌肉（Tamaki和Akatsuka， Anat.Rec.1994）。因此，CBF的存在可能会干扰整个肌肉组织化学和功能特性之间的相关性。老年大鼠的抽搐和破伤风特性也比对照大鼠慢。对照组大鼠在100Hz的刺激频率下均可获得完全破伤风。然而，一些老年大鼠不能跟随超过50Hz的刺激。这表明，考虑衰老对运动功能的影响是一个非常重要和有趣的事实。总之，这种“实验动物运动功能分析的新改进方法”可用于定量测定各种实验动物的运动功能。

项目成果

Tetsuro TAMAKI: "Characteristics of compensatory hypertrophied muscle in the rats : II.Comparison of histochemical and functional properties." The Anatomical Record. 246. 335-342 (1996)

Tetsuro TAMAKI：“大鼠代偿性肥大肌肉的特征：II.组织化学和功能特性的比较。”

Tetsuro Tamaki: "Characteristics of compensatory hypertrophied muscle in the rat:I.Electron microscopic and immunohistochemical studies." The Anatomical Record. 246. 325-334 (1996)

Tetsuro Tamaki：“大鼠代偿性肥大肌肉的特征：I.电子显微镜和免疫组织化学研究。”

Tetsuro TAMAKI: "Characteristics of compensatory hypertrophied muscle in the rats : I.Electron microscopic and immunohistochemical studies." The Anatomical Record. 246. 325-334 (1996)

Tetsuro TAMAKI：“大鼠代偿性肥大肌肉的特征：I.电子显微镜和免疫组织化学研究。”

Tetsuro Tamaki: "Characteristics of compensatory hypertrophied muscle in the rat:II.Comparison of histochemical and functional properties." The Anatomical Record. 246. 335-342 (1996)

Tetsuro Tamaki：“大鼠代偿性肥大肌肉的特征：II.组织化学和功能特性的比较。”

Tetsuro Tamaki: "Characteristics of compensatory hypertrophied muscle in the rat : I.Electron microscopic and immunohistochemical studies." The Anatomical Record. 246. 325-334 (1996)

Tetsuro Tamaki：“大鼠代偿性肥大肌肉的特征：I.电子显微镜和免疫组织化学研究。”