Expression of a splice variant of choline acetyltransferase in magnocellular neurons of the tuberomammillary nucleus of rats

胆碱乙酰转移酶剪接变体在大鼠结节乳头核大细胞神经元中的表达

• 批准号: 12480225
• 负责人: KIMURA Hisoshi
• 金额: $ 9.34万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480225/
• 关键词: Cholinergic; Hypothalamus; Neurotransmitter; Immunohistochemistry; Colooalization; Histidine decarboxylase; Adenosine deaminase; RT-PCR; Alternative splicing; アセチルコリン; コリン神経; 隆起乳頭体核; スプライシング; mRNA; 遺伝子発現

A splice variant of choline acetyltransferase mRNA has recently been identified in rat pterygopalatine ganglion. An antiserum against this variant protein (pChAT) stained peripheral cholinergic neurons. In the present study, we examined the immunohistochemical localization of pChAT in rat brain. Although the antiserum failed to stain known cholinergic neurons in the brain, magnocellular neurons in the tuberomammillary nucleus (TMN) of the posterior hypothalamus were stained with the antiserum. RT- PCR experiments confirmed the expression of pChAT mRNA in rat posterior hypothalamus. The distribution pattern of pChAT-positive neurons in the nucleus was similar to that positive for histidine decarboxylase (HDC) and adenosine deaminase (ADA). Double immunohistochemical staining in rats pretreated with colchicine indicated mat virtually all pChAT-positive cells contained ADA. Some pChAT-positive cells in the nucleus were retrogradely labeled following the injection of cholera-toxin B subunit into the cerebral cortex. These results suggest the existence of a presumed corticipetal cholinergic pathway ascending from the TMN.

最近在大鼠翼腭神经节中发现了胆碱乙酰转移酶mRNA的剪接变体。抗这种变异蛋白（pChAT）的抗血清染色外周胆碱能神经元。在本研究中，我们研究了pChAT在大鼠脑内的免疫组织化学定位。虽然抗血清未能染色已知的胆碱能神经元在大脑中，大细胞神经元在结节乳头核（TMN）的后下丘脑染色的抗血清。RT-PCR实验证实pChAT mRNA在大鼠下丘脑后部有表达. pChAT阳性神经元在核内的分布模式与组氨酸脱羧酶（HDC）和腺苷脱氨酶（ADA）阳性神经元的分布模式相似。在用秋水仙碱预处理的大鼠中，双重免疫组织化学染色表明几乎所有pChAT阳性细胞都含有ADA。将霍乱毒素B亚单位注入大脑皮质后，可逆行标记细胞核内的pChAT阳性细胞。这些结果表明，存在一个假定的皮质胆碱能通路上升的TMN。

项目成果

Matsukawa N: "Increased expression of hippocampal cholinergic neurostimulating peptide-related compounds and their messenger mas in the hippocampus of aged senescence-accelerated mice."Neuroscience. 88. 79-92 (1999)

Matsukawa N：“衰老加速小鼠海马中海马胆碱能神经刺激肽相关化合物及其信使的表达增加。”神经科学。

Kitano H: "Expression of inducible nitric oxide synthase in human thyroid papillary carcinoma."Thyroid. 9. 113-117 (1999)

Kitano H：“诱导型一氧化氮合酶在人甲状腺乳头状癌中的表达。”甲状腺。

Tooyama I, Abe H, Renda TG, Kimura H: "Production and immunohitochemical application of antiserum against Tyr-D-Ala-Phe, a N-terminal tripeptide common to dermorphin/deltorphin family"Peptides. 21. 1649-1655 (2000)

Tooyama I、Abe H、Renda TG、Kimura H：“针对 Tyr-D-Ala-Phe（dermorphin/deltorphin 家族常见的 N 端三肽）的抗血清的生产和免疫组织化学应用”肽。

Kiriyama K, Hirouchi M, Kimura H: "Neurochemical and molecular phamacological aspects of the GABAB receptor"Neurochem.Res.. 25. 1233-1239 (2000)

Kiriyama K、Hirouchi M、Kimura H：“GABAB 受体的神经化学和分子药理学方面”Neurochem.Res.. 25. 1233-1239 (2000)

Kuriyama K., Hirouchi M., Kimura H.: "Neurochemical and molecular pharmacological aspects of the GABAB receptor"Neurochem. Res.. 25. 1233-1239 (2000)

Kuriyama K.、Hirouchi M.、Kimura H.：“GABAB 受体的神经化学和分子药理学方面”Neurochem。