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Sweat measurement technique using the electrical conductivity method applicale for QSART

使用适用于 QSART 的电导率法的汗液测量技术

基本信息

批准号：
12480259
负责人：
TOGAWA Tatsuo
金额：
$ 2.75万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Shamsuddin et al. have reported that sweating from a sweat gland can be recorded continuously using a technique in which an ion-free solution is perfused to a small region of the skin, with the secreted sweat being detected by changes in electrical conductivity. We have improved Shamsuddin's electrical conductivity cell, and developed a measuring system that can simultaneously record sweating from up to four independent sweat glands. The sudomotor axon reflex was observed using a ring-shaped acetylcholine well placed coaxially in relation to the conductivity cell, and a DC current applied from the well to the counter electrode. The direct effect of acetylcholine on the sweat glands was observed by perfusing acetylcholine solution to a cell attached to the forearm, and then applying a DC current from an electrode in the cell to a counter electrode attached to the body. In single sweat gland measurements, simultaneous recording of two or more sweat glands could be carried out on all subjects, and a subject sweating from four sweat glands could be recorded repeatedly. In most observations, the sweat responses recorded from different sweat glands were different in amplitude and in waveform. However, similar sweat response patterns were occasionally observed. The direct effect of acetylcholine on a sweat gland was recorded after introducing this substance by applying current for about one minute. Pulsatile sweat responses were observed repeatedly, and the responses declined within a period of about 10 min. Axon reflex sweating could be observed, and pulsatile sweat patterns were also recognised.

Shamsuddin等人报道，使用一种技术可以连续记录汗腺的出汗，该技术将无离子溶液灌注到皮肤的一小块区域，通过电导率的变化来检测分泌的汗液。我们改进了Shamsuddin的电导细胞，并开发了一种测量系统，可以同时记录多达四个独立汗腺的出汗情况。使用环状乙酰胆碱与电导率细胞同轴放置，并从电导率细胞向对电极施加直流电流，观察了sudomotor轴突反射。通过将乙酰胆碱溶液灌注到附着在前臂的细胞中，然后从细胞内的电极向附着在身体上的反电极施加直流电，观察了乙酰胆碱对汗腺的直接作用。在单次汗腺测量中，可以同时记录所有受试者的两个或多个汗腺，并且可以重复记录受试者的四个汗腺的出汗。在大多数观察中，不同汗腺记录的汗液反应在振幅和波形上是不同的。然而，偶尔也会观察到类似的出汗反应模式。通过施加电流约一分钟后，记录了乙酰胆碱对汗腺的直接作用。反复观察脉动性出汗反应，反应在大约10分钟内下降。可以观察到轴突反射性出汗，也可以识别出脉动性出汗模式。