Sweat measurement technique using the electrical conductivity method applicale for QSART

Shamsuddin等人已经报道，可以使用将无离子溶液灌注到皮肤的小区域的技术连续记录来自汗腺的出汗，其中通过电导率的变化检测分泌的汗液。我们改进了Shamsuddin的电导池，并开发了一种测量系统，可以同时记录多达四个独立汗腺的出汗。使用相对于电导池同轴放置的环形乙酰胆碱以及从该井施加到对电极的DC电流来观察汗动轴突反射。乙酰胆碱对汗腺的直接作用是通过将乙酰胆碱溶液灌注到附着于前臂的细胞中，然后从细胞中的电极向附着于身体的反电极施加DC电流来观察的。在单个汗腺测量中，可以对所有受试者同时记录两个或更多个汗腺，并且可以重复记录受试者从四个汗腺出汗。在大多数观察中，从不同汗腺记录的汗液反应在幅度和波形上是不同的。然而，偶尔观察到类似的汗液反应模式。在通过施加电流约1分钟引入该物质后，记录乙酰胆碱对汗腺的直接影响。反复观察到搏动性汗液反应，并在约10分钟的时间内下降的反应。轴突反射出汗可以观察到，和搏动性汗液模式也被认可。

Shamsuddin et al. have reported that sweating from a sweat gland can be recorded continuously using a technique in which an ion-free solution is perfused to a small region of the skin, with the secreted sweat being detected by changes in electrical conductivity. We have improved Shamsuddin's electrical conductivity cell, and developed a measuring system that can simultaneously record sweating from up to four independent sweat glands. The sudomotor axon reflex was observed using a ring-shaped acetylcholine well placed coaxially in relation to the conductivity cell, and a DC current applied from the well to the counter electrode. The direct effect of acetylcholine on the sweat glands was observed by perfusing acetylcholine solution to a cell attached to the forearm, and then applying a DC current from an electrode in the cell to a counter electrode attached to the body. In single sweat gland measurements, simultaneous recording of two or more sweat glands could be carried out on all subjects, and a subject sweating from four sweat glands could be recorded repeatedly. In most observations, the sweat responses recorded from different sweat glands were different in amplitude and in waveform. However, similar sweat response patterns were occasionally observed. The direct effect of acetylcholine on a sweat gland was recorded after introducing this substance by applying current for about one minute. Pulsatile sweat responses were observed repeatedly, and the responses declined within a period of about 10 min. Axon reflex sweating could be observed, and pulsatile sweat patterns were also recognised.