Development of new migration analysis of micro-particles by using electromagnetophoretic buoyancy

利用电磁泳浮力进行微粒迁移分析的新方法的开发

基本信息

批准号：
12554033
负责人：
WATARAI Hitoshi
金额：
$ 8.58万
依托单位：
OSAKA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Development of new method for separation and characterization of micro-particles or colloid particles in water of environmental and biological interests are highly required in these modern environmental and biological sciences. We noticed the electromagnetophoretic buoyancy as a powerful new migration force and we developed some principles for new migration methods of micro-articles in water.1) Development of capillary electromagnetophoresisWhen both electric current and magnetic field are applied orthogonarly to the Solution in a capillary, the electromagnetophoretic buoyancy is produced in the solution, which can migrate micro-particles in the solution to the direction perpendicular to the current. It worked even under flow condition. By using this principle, surface potential of particles have been estimated.2) Measurement of desorption force of microparticles in the pN levelBy controlling the electromagnetophoretic buoyancy, the adsorption force of micro-particles with the silica capillary wall in solution was measured. Effect of surfactant on the adsorption force was also determined. The advantages of this method, in comparison with AFM, are that pN force can be detected in the closed system by rather simple principle and devise.3) Development of electromagnetophoretic chromatographyBy applying profile controlled AC current through the capillary under magnetic field, we developed new principle for a chromatographic separation of particles.

在这些现代的环境和生物科学中，需要开发微粒或生物学利益水中微粒或胶体颗粒的新方法。 We noticed the electromagnetophoretic buoyancy as a powerful new migration force and we developed some principles for new migration methods of micro-articles in water.1) Development of capillary electromagnetophoresisWhen both electric current and magnetic field are applied orthogonarly to the Solution in a capillary, the electromagnetophoretic buoyancy is produced in the solution, which can migrate micro-particles in the solution to the direction垂直于电流。它甚至在流动状态下工作。通过使用该原理，已经估计了颗粒的表面电位。2）测量控制电磁酚浮力的Pn级别的微粒的解吸力，测量微粒在溶液中用硅胶壁的吸附力。还确定了表面活性剂对吸附力的影响。与AFM相比，该方法的优点是可以通过相当简单的原理和设计在封闭的系统中检测到PN力。3）开发电磁噬菌学色谱法，以通过磁场在毛细管下使用毛细管电流，我们开发了新的原理，以进行粒子的色谱分离。