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Development of new migration analysis of micro-particles by using electromagnetophoretic buoyancy

利用电磁泳浮力进行微粒迁移分析的新方法的开发

基本信息

批准号：
12554033
负责人：
WATARAI Hitoshi
金额：
$ 8.58万
依托单位：
OSAKA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Development of new method for separation and characterization of micro-particles or colloid particles in water of environmental and biological interests are highly required in these modern environmental and biological sciences. We noticed the electromagnetophoretic buoyancy as a powerful new migration force and we developed some principles for new migration methods of micro-articles in water.1) Development of capillary electromagnetophoresisWhen both electric current and magnetic field are applied orthogonarly to the Solution in a capillary, the electromagnetophoretic buoyancy is produced in the solution, which can migrate micro-particles in the solution to the direction perpendicular to the current. It worked even under flow condition. By using this principle, surface potential of particles have been estimated.2) Measurement of desorption force of microparticles in the pN levelBy controlling the electromagnetophoretic buoyancy, the adsorption force of micro-particles with the silica capillary wall in solution was measured. Effect of surfactant on the adsorption force was also determined. The advantages of this method, in comparison with AFM, are that pN force can be detected in the closed system by rather simple principle and devise.3) Development of electromagnetophoretic chromatographyBy applying profile controlled AC current through the capillary under magnetic field, we developed new principle for a chromatographic separation of particles.

现代环境和生物科学迫切需要开发新的方法来分离和表征具有环境和生物学意义的水中微颗粒或胶体颗粒。我们注意到电磁泳浮力是一种强大的新型迁移力，并提出了微颗粒在水中迁移新方法的一些原理。1)毛细管电磁泳体的发展当电流和磁场正交作用于毛细管中的溶液时，溶液中产生电磁泳体浮力，使溶液中的微粒向垂直于电流的方向迁移。在流动工况下也能正常工作。利用这一原理，估计了粒子的表面势。2) pN级微颗粒解吸力的测定通过控制电磁浮浮力，测定了微颗粒在溶液中对二氧化硅毛细管壁的吸附力。测定了表面活性剂对吸附力的影响。与原子力显微镜相比，该方法的优点是原理和设计简单，可以在封闭系统中检测到pN力。3)电磁电泳色谱的发展：在磁场作用下，通过毛细管施加剖面控制的交流电流，建立了一种新的颗粒色谱分离原理。