Construction of new host-vector systems of the production of foreign proteins using a variety of protein targeting signals.

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1. We developed a new transformation system using multiple auxotrophic marker genes for the fission yeast Schizosaccharomyces pombe. This new transformation system would be an useful tool for molecular genetic studies of S. pombe where multiple recombinant modifications or multiple mutations are needed.2. We have isolated many genes that are required for protein secretion and sorting in fission yeast. Using a variety of protein targeing signals and mutants, we constructed new types of host-vector systems to produce foreign proteins.3. The glycoproteins of S. pombe contain a large amount of galactose in addition to mannose, indicating that S. pombe is equipped with mechanisms for galactosylation of glycoprotein, like mammalian cells. To elucidate the physiological role of galactosylation, we isolated several S. pombe mutants defective in protein galactosylation. We elucidate that the galactosylation of protein is required for the maintenance of normal cell shape, sexual agglutination, tolerance toward various drugs, and non-sexual flocculation in S. pombe.