Application of SNPs-detection using DNA chips to personal identification and examination of cause of sudden death

DNA芯片SNP检测在个人身份识别及猝死原因检查中的应用

• 批准号: 12557042
• 负责人: ISHITANI Akiko
• 金额: $ 2.69万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557042/
• 关键词: SNPs; susceptible gene; HLA; KIR gene; polymorphism; DNA chip; personal identification; unexplained sudden death; sudden death

【○!1】 We have identified more than 10000 SNPs in all HLA region (Chromosome 6) and have designed 160 sets of the primers for typing the SNPs by SSP (Sequence Specific Primer) method.【○!2】 We have studied polymorphism of KIR (Killer Immunoglobulin-like Receptor) gene (Chromosome 19) and identified 24 genes and 9 haplotypes including 2 new genes and 7 new haplotypes.【○!3】 We have collected 100 DNA samples of each of healthy Japanese, Rheumatoid Arthritis-patients and pollen allergy-patients with informed consent, and typed 160 SNPs in HLA region. The datas are started to analyse.【○!4】 We have studied the detection methods of SNPs using DNA chips and found that M13 tailed labelling method made a more clear and correct results.【○!5】 We have examined the application of SNPs in HLA region, KIR genes and ABO genes for paternity test and found that they made high percentage of paternity. We are going to find more SNPs in more chromosomes for paternity test or personal identification.

【○！1】我们在HLA全区（6号染色体）鉴定出超过10000个SNP，并通过SSP（Sequence Specific Primer）方法设计了160套SNP分型引物。【○！2】我们研究了KIR（杀伤性免疫球蛋白样受体）基因（19号染色体）的多态性，鉴定了24个基因和9个单倍型，包括 2个新基因和7个新单倍型。【○！3】我们在知情同意的情况下收集了日本健康人、类风湿性关节炎患者和花粉过敏患者的每人100个DNA样本，并对HLA区域的160个SNP进行了分型。开始分析数据。【○！4】我们研究了利用DNA芯片检测SNP的方法，发现M13尾标记法的结果更加清晰、正确。【○！5】我们研究了HLA区、KIR基因和ABO基因的SNP在亲子鉴定中的应用，发现其亲子鉴定率很高。我们将在更多的染色体中找到更多的SNP，用于亲子鉴定或个人鉴定。

项目成果

Akiko Ishitani: "Analisis of Natural killer immunoglobulin-like receptor (KIR) gene polymorphism"DNA Polymorphism. 9. 158-160 (2001)

Akiko Ishitani：“自然杀伤免疫球蛋白样受体（KIR）基因多态性分析”DNA 多态性。

Akiko Ishitani: "Molecules concerned with the fractions of NK cells-NKG2 (A/B, C, D, E/H, F)"Clinical Immunology. 34(Suppl.19). 263-268 (2000)

Akiko Ishitani：“与 NK 细胞组分有关的分子 - NKG2 (A/B、C、D、E/H、F)”临床免疫学。

石谷昭子: "Natural immunoglobulin-like receptor (KIR)遺伝子の多型解析"DNA多型. 9. 158-160 (2001)

Akiko Ishitani：“天然免疫球蛋白样受体（KIR）基因的多态性分析”DNA多态性。9. 158-160（2001）。

石谷昭子: "NK細胞の機能発現に関わる分子-NKG2(A/B,C,D,E/H,F)"臨床免疫. 34(Suppl 19). 263-268 (2000)

Akiko Ishitani：“参与 NK 细胞功能表达的分子 - NKG2 (A/B、C、D、E/H、F)”临床免疫学 34（增刊 19）。

石谷昭子: "Natural immunoglobulin-like receptor(KIR)遺伝子の多型解析"DNA多型. 9. 158-160 (2001)

Akiko Ishitani：“天然免疫球蛋白样受体（KIR）基因的多态性分析”DNA多态性。9. 158-160（2001）。