Application of SNPs-detection using DNA chips to personal identification and examination of cause of sudden death

○！1]我们在HLA的所有区域（6号染色体）中鉴定了10000多个SNPs，并设计了160套引物，用SSP（Sequence Specific Primer）方法对SNPs进行分型。○！2]本研究对19号染色体KIR基因的多态性进行了研究，共鉴定出24个基因和9种单倍型，其中包括2个新基因和7种新单倍型。○！3]我们收集了100例健康日本人、风湿性关节炎患者和花粉过敏患者的DNA样本，并对HLA区域的160个SNP进行了分型。开始对数据进行分析。○！4]我们对DNA芯片检测SNP的方法进行了研究，发现M13加尾标记法的检测结果更加清晰、准确。○！5]我们研究了HLA区域、KIR基因和ABO基因的SNPs在亲子鉴定中的应用，发现它们具有较高的亲子鉴定率。我们将在更多的染色体上发现更多的SNPs，用于亲子鉴定或个人识别。

【○!1】 We have identified more than 10000 SNPs in all HLA region (Chromosome 6) and have designed 160 sets of the primers for typing the SNPs by SSP (Sequence Specific Primer) method.【○!2】 We have studied polymorphism of KIR (Killer Immunoglobulin-like Receptor) gene (Chromosome 19) and identified 24 genes and 9 haplotypes including 2 new genes and 7 new haplotypes.【○!3】 We have collected 100 DNA samples of each of healthy Japanese, Rheumatoid Arthritis-patients and pollen allergy-patients with informed consent, and typed 160 SNPs in HLA region. The datas are started to analyse.【○!4】 We have studied the detection methods of SNPs using DNA chips and found that M13 tailed labelling method made a more clear and correct results.【○!5】 We have examined the application of SNPs in HLA region, KIR genes and ABO genes for paternity test and found that they made high percentage of paternity. We are going to find more SNPs in more chromosomes for paternity test or personal identification.