The potential role of HLA-G on transplantation immunity - examination of increasing recent reports -

近年来，有越来越多的报道认为实体器官和造血干细胞移植后血液中的人类白细胞抗原-G表达与移植成功有关。为了解决这些发现，我们重新检测了肾脏、肝脏和造血干细胞移植中的人类白细胞抗原-G蛋白表达。这些研究显示移植前或移植后患者外周血单个核细胞上有人类白细胞抗原-G的表达，但没有发现人类白细胞抗原-G的表达与移植成功之间的显著关联。我们还用ELISA法检测了患者血浆中的人类白细胞抗原-G蛋白，虽然用免疫印迹法不能确定这些样本中的人类白细胞抗原-G蛋白。

Recently, the number of reports suggesting that HLA-G expression in blood after solid organ and hematopoietic stem cells transplants being associated with successful transplantation has been increasing. To address these findings, we reexamined HLA-G protein expression in kidney, liver and hematopoietic stem cell transplantation. These studies revealed HLA-G expression on peripheral mononuclear cells from patients before or after transplantation, but found no significant association of HLA-G expression with successful transplantation. We also detected HLA-G protein in plasma from patients by ELISA, although we were unable to confirm HLA-G protein in these samples by western analysis.