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Immunological control by the interaction between HLA-E and HLA-G in fetal-maternal interface

通过胎母界面中 HLA-E 和 HLA-G 之间的相互作用进行免疫控制

基本信息

批准号：
11671643
负责人：
ISHITANI Akiko
金额：
$ 2.3万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671643/
关键词：
HLA-E HLA-F HLA-G cytokine signal peptide placenta trophoblast NK receptor decidua

项目摘要

l) The effects of HLA-G expression of trophoblast cells on cytokine secretion by decidual mononuclea cells were examined. The HLA-G expression decreased the secretion of Th1-type cytokine, IFN γ and TNF α, and increased Th2-type cytokine, IL4 and IL10 suggesting that one of the main roles of HLA-G is to control cytokine network in placenta.2) Although it had been thought that trophoblast cells expressed only HLA-G with neither any other class Ia nor class II HLA antigen, we showed HLA-E and HLA-F are also expressed on trophoblast using anti-HLA-E and anti-HLA-F monoclonal antibodies we had made.3) Correlation between the expression of HLA-G and -E on trophoblasts and the cause of preeclampsia was examined. Several data were reported suggesting that one of the main causes of preeclampsia might bethe loss of the HLA-G expression on the trophoblasts. Although, we also found the partial lack of the expression of HLA-G on normal placenta . And those trophoblasts without the expression of HLA-G also did not express HLA-E, were surrounded with fiblin as same as trophoblasts of preeclampsia, and were not stained with eosin, suggesting that those cells were inert or dying already. That means the lack of expression of HLA-G on trophoblasts may be the result of the desease rather than the cause.

L)研究了滋养层细胞HLAG表达对蜕膜单核细胞分泌细胞因子的影响。HLAG的表达减少了Th1型细胞因子干扰素γ和肿瘤坏死因子α的分泌，增加了Th2型细胞因子IL4和IL10的分泌，提示HLAG的主要作用之一是控制胎盘的细胞因子网络。2)虽然以往认为滋养层细胞只表达HLAG，而没有其他Ia类和II类抗原，但利用我们制备的抗HLAE和抗HLAF的单抗，我们发现滋养层细胞上也表达了HLAE和HLAF。多项研究表明，子痫前期的主要原因之一可能是滋养层细胞表面人类白细胞抗原-G的表达缺失。尽管如此，我们也发现正常胎盘组织中部分缺失了人类白细胞抗原-G的表达。而不表达HLAG的滋养层细胞也不表达HLAE，与子痫前期滋养层细胞一样被纤维蛋白包裹，未见伊红染色，提示这些细胞已经处于惰性或死亡状态。这意味着滋养层细胞表面缺乏HLA-G的表达可能是疾病的结果而不是原因。