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Analysis of estrogenic activity of resin composites in vitro and in vivo

树脂复合材料体内外雌激素活性分析

基本信息

批准号：
12557160
负责人：
TARUMI Hisashi
金额：
$ 8.51万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Estrogenicity of commercial composites, which contain various monomers and additives, has not yet been fully elucidated. In this study, the estrogenic activities of eluates obtained from 24 composites were examined in vitro (reporter gene assay). The estrogenicity of 18 chemicals identified from the composites was also assessed by a reporter gene assay. Furthermore, the estrogenic activity of the chemicals, which were confirmed to have estrogenic activity in vitro, was examined in vivo (uterotrophic assay).Among 24 composites, six products were estrogenic in vitro, although none of the specimens contained bisphenol-A. Moreover, one photostabilizer (2-hydroxy-4-methoxy-benzophenone, HMBP), one photoinitiator (2,2-dimethoxy-2-phenyl-acetophenone, DMPA), and one inhibitor (2,6-di-tert-butyl-ρ-cresol, BHT) showed significant estrogenic activities among 18 chemicals in vitro. The concentrations of HMBP in 4 estrogenic eluates were greater than the minimum concentration to show estrogenicity. For other 2 estrogenic concentration. In addition, BHT was not identified in any of the eluates. Therefore, the estrogenic activites of composites observed in vitro were associated with the elution of HMBP or DMPA. On the contrary, neither HMBP nor DMPA showed estrogenicity in vivo. These results suggest that the possibilities of the chemicals eluted from resin composites mimicking natural steroid hormones are negligible.

含有各种单体和添加剂的商业复合材料的雌激素活性尚未完全阐明。在这项研究中，对24种复合物的洗脱液进行了体外雌激素活性检测（报告基因测定）。还通过报告基因测定评估了从复合物中鉴定的18种化学物质的雌激素活性。此外，在体外证实具有雌激素活性的化合物，在体内进行了检查（子宫肥大试验）。在24个复合物中，6个产品在体外具有雌激素活性，虽然没有标本含有双酚A。在18种化合物中，1种光稳定剂（2-羟基-4-甲氧基二苯甲酮，HMBP）、1种光引发剂（2，2-二甲氧基-2-苯基苯乙酮，DMPA）和1种抑制剂（2，6-二叔丁基对甲酚，BHT）显示出明显的雌激素样活性。4种雌激素洗脱液中HMBP的浓度均大于显示雌激素活性的最低浓度。对于其他2种雌激素浓度。此外，在任何洗脱液中均未鉴别出BHT。因此，体外观察到的复合物的雌激素活性与HMBP或DMPA的洗脱有关。相反，HMBP和DMPA在体内均不显示雌激素样活性。这些结果表明，从树脂复合材料中洗脱的化学物质模仿天然类固醇激素的可能性是微不足道的。