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Risk assessment of root canal disinfectants in terms of endocrine-disruption and development of their substitutes

根管消毒剂内分泌干扰风险评估及其替代品开发

基本信息

批准号：
13671998
负责人：
TARUMI Hisashi
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671998/
关键词：
Root canal disinfectant endocrine-disrupting chemicals environmental hormone estlvgenicity reporter gene assay uterotrophic assay in vitro in vivo

项目摘要

In this study, estrogenic activities of four root canal disinfectants; Formalin/Cresol (FC), Formalin/Guaiacol (FG), p-Chlorophenol/Guaiacol (Methocol^【R!○】), and Guaiacol (Creodon^【R!○】), and their components were investigated in vitro and in vivo.By in vitro reporter gene assay, Methocol was found to demonstrate estrogenic activity at 10μg/mL or greater, and p-Chlorophenol and p-Cresol were estrogenic at 50 μM and over. For FC and FG, the activity could not be determined as the cells were killed due to the toxicity of the specimens even at the least concentration tested. The estrogenicity shown by Methocol is considered to be dependent upon p-Chlorophenol, and it is possible that FC exhibits estrogenic activity as it contains p-Cresol.In vivo estrogenicities of the four disinfectants and the components were studied by uterotrophic assay using ovariectomized rats. Disinfectants were administered at 15 μL/kg/day and, the componentsat 50 mg/kg/day. For any of the specimens, no change in the weight or morphology of ovary was observed, indicating that these chemicals were not estrogenic in vivo.These results indicate that estrogenicities of root canal disinfectants tested were negligible under in vivo condition, although some of their components showed estrogenic-activity by in vitro assays.

在这项研究中，四种根管消毒剂的雌激素活性；福尔马林/甲酚（FC）、福尔马林/愈创木酚（FG）、对氯酚/愈创木酚（Methocol^【R！○】）和愈创木酚（Creodon^【R！○】）及其成分在体外和体内进行了研究。通过体外报告基因检测，发现Methocol在10μg/mL时表现出雌激素活性或更高，对氯苯酚和对甲酚在 50 μM 及以上时具有雌激素作用。对于 FC 和 FG，即使在最低测试浓度下，由于样本的毒性，细胞也会被杀死，因此无法确定其活性。 Methocol显示的雌激素活性被认为依赖于对氯苯酚，并且FC可能由于含有对甲酚而表现出雌激素活性。使用卵巢切除的大鼠通过子宫营养测定法研究了四种消毒剂及其成分的体内雌激素活性。消毒剂的施用量为 15 μL/kg/天，成分的施用量为 50 mg/kg/天。对于任何标本，都没有观察到卵巢重量或形态的变化，表明这些化学物质在体内不具有雌激素作用。这些结果表明，尽管体外检测显示根管消毒剂的某些成分显示出雌激素活性，但在体内条件下，所测试的根管消毒剂的雌激素作用可以忽略不计。