Analysis of genetic risk factors in periodontal diseses ― Effect of cellular aging on damage of cells ―

1.用Southern blotting方法分析正常和唐氏综合征患者成纤维细胞的端粒DNA长度。经5次细胞复制后，发现唐氏综合征成纤维细胞的端粒DNA长度明显短于健康成纤维细胞。唐氏综合征患者来源的成纤维细胞表达干扰素α/β(IMF-α/β)受体亚单位IFNAR1，比健康患者来源的成纤维细胞表达更强。转录因子STAT1是位于IMF受体下游的转录因子之一，可被干扰素-γ激活。Western blotting结果显示，干扰素-γ作用后，唐氏成纤维细胞中活化的STAT1的表达强于健康成纤维细胞。然而，在有抗INF-β抗体存在的情况下，经INF-γ处理后，活化的STAT1被减少。白细胞介素6(IL-6)是由干扰素-γ诱导产生的一种炎性细胞因子。ELISA法显示，经干扰素-γ处理后，唐氏成纤维细胞IL-6的产生明显高于健康成纤维细胞。

1. Telomere DNA length of human fibroblasts derived from healthy and Down syndrome patients was analyzed by Southern blotting. After 5 times cell replication, it was shown that telomere DNA length was more short in fibroblasts from Down syndrome than from healty fibroblasts.2. Fibroblasts derived from Down syndrome patients expressed interferon alpha/ beta (IMF-α/β) receptor subunit, IFNAR1, stronger than fibroblasts from healthy patients.3. STAT1, one of transcriptional factors in the downstream of IMF receptor, was activated by the treatment of INF- γ. Western blotting demonstrated that activated STAT1 in Down fibroblasts was expressed stronger than in healthy fibroblasts after the treatment of INF-γ. However, in the presence of anti-INF-β antibody, activated STAT1 was reduced by the treatment of INF-γ.4. Interleukin-6 (IL-6), one of inflammatory cytokines, was induced by the treatment of INF -γ. ELISA demonstrated that IL-6 production in Down fibroblasts increased more abundantly than in healthy fibroblasts by the treatment of INF- γ.