Gene transfection by cationic liposome with a cationic cholesterol derivative

我们将展示具有新型阳离子胆固醇衍生物的阳离子脂质体，这是我们小组开发的一种基因转染的新策略，以及目前公认的基因转染的分子机制。用共聚焦激光扫描显微镜和原子力显微镜研究阳离子脂质体转染基因的分子机制。正如所描述的那样，共聚焦激光扫描显微镜和原子力显微镜结果都主张在阳离子脂质体介导的基因转染中参与连续的三个步骤：内部的内化lipoplexes到目标细胞,endosome-lysosome融合,DNA被从脂质体中释放出来,走向目标细胞的细胞核和微管组织显然参与贩运溶酶体转染外源基因。此外，我们的实验还显示了基因转染的几个重要策略，即使用由生物表面素制成的脂质体和通过激活膜结合受体分子来有效地进行基因转染。

We will demonstrate interesting cationic liposomes with novel cationic cholesterol derivatives, a new strategy in gene transfection developed by our group and the presently accepted molecular mechanism of gene transfection. Use of confocal laser scanning microscopy and atomic force microscopy in elucidating the molecular mechanism of gene transfection by cationic liposomes is also showed using examples from our own work. As delineated, both the confocal laser scanning microscopic and the atomic force microscopic results advocate for the involvement of the sequential three steps in gene transfection mediated by the cationic liposomes: endocytotic internalization of the lipoplexes into the target cells, endosome-lysosome fusion whereby the DNA gets released from the liposomes and moves towards the nucleus of the target cells and microtubule organization apparently involved in trafficking the transfected foreign genes to lysosomes. Furthermore, our experiment also shows couple of important strategies in gene transfection namely, use of liposomes made from biosurfactans and harnessing efficient gene transfection by activating the membrane-bound receptor molecules.