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A novel development and utilization of congenic rat strains with autoimmune type 1 diabetes mellitus and Hashimoto' diesease

自身免疫1型糖尿病桥本病同系大鼠品系的新开发利用

基本信息

批准号：
12558096
负责人：
KANAZAWA Masao
金额：
$ 6.34万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12558096/
关键词：
Hashimoto' Disease BB W Rat Thyroiditis RT1 class II ラット 1型糖尿病膵島炎 T_3, T_4 MHCコンジェニック T_3,T_4

项目摘要

The RT1 class II area is u/u in BB/W rats, insulitis occurs, resulting type 1 DM. When the area is a/a, thyoiditis occurs, resulting in Hashimoto's disease.In the present study, a large number of rats developing Hashimoto's disease and rats with hereditary stability were prepared. The following rats were prepared in the present study:Type u/u: 82 males and 76 females, totaling 158 rats.Type u/a: 94 males and 111 females, totaling 205 rats.Type a/a: 28 males and 32 females, totaling 60 rats.There were 423 rats in total.Hereditary stabilization was induced by repeated back-crossing. In the 10th generation, all chromosomes other than chromosome No.20 were fixed to a homogeneous genotype. Chromosome No.20, which includes the area of the major histocompatibility complex (MHC), still showed a heterogeneous genotype.The type a/a rats developed thuroiditis at an incidence of approximately 90% about 210 days after the preparation. The blood level of thyroid hormones were low in these rats, but the pathological findings of thyroiditis were not completely consistent with the blood level of the thyroid hormones.In the future, a stable supply of thyroiditis model rats, by the development of rats with fixation of chromosome No.20 as well to the homogeneous genotype, will become increasingly necessary.

BB/W大鼠RT1ⅱ类区域为u/u时，发生胰岛素炎，导致1型糖尿病。当该区域为a/a时，发生甲状腺炎，导致桥本病。本研究制备了大量的桥本病大鼠和遗传稳定大鼠。本研究准备的大鼠如下：u/u型：雄性82只，雌性76只，共158只。u/a型：雄性94只，雌性111只，共205只。a/a型：雄性28只，雌性32只，共60只。共423只大鼠。重复回交诱导遗传稳定。在第10代，除第20号染色体外，其余染色体均固定为均匀基因型。包括主要组织相容性复合体（MHC）区域的20号染色体仍表现为异质基因型。a/a型大鼠在制备后约210天发生甲状腺炎，发生率约为90%。血中甲状腺激素水平较低，但甲状腺炎的病理表现与血中甲状腺激素水平不完全一致。在未来，通过20号染色体固定大鼠和同质基因型大鼠的发展来稳定供应甲状腺炎模型大鼠将变得越来越必要。