Studies on genetic changes in interspecific hybridization of primates

灵长类种间杂交遗传变化的研究

• 批准号: 13440256
• 负责人: KAWAMOTO Yoshi
• 金额: $ 8万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440256/
• 关键词: INTERSPECIFIC HYBRID; JAPANESE MACAQUES; TAIWANESE MACAQUES; RHESUS MACAQUES; INTRODUCED SPECIES; NUCLEAR DNA.; MITOCHONDRIAL DNA; AFLP; 異種交雑; Y染色体遺伝子; 血液タンパク質; マイクロサテライト遺伝子; 種間交雑; チャイロキツネザル; TSPY遺伝子; 種判別

A macaque population produced by the hybridization of native Japanese macaques and introduced Taiwanese macaques in Wakayama was studied for its genetic features.. Using nuclear, Y chromosome and mitochondrial DNA markers, it was revealed that the hybridization had occurred through interbreed of group females with immigrant Japanese males by producing fertile offspring and that relative gene frequencies of Japanese macaques increased rapidly in the hybrid population. Dispersal of exotic genes from the hybrid population by adult male transfer was investigated by assessing geographic distribution of Y chromosome specific markers. There was no evidence of gene flow from the introduced population to those in the neighboring provinces. A diagnostic nuclear marker of natural resistance-associated macrophage protein 1 (NRAMPI) was newly developed for the assessment of hybridization. Preliminary results were also obtained for the applicability of amplified fragment length polymorphism (AFLP) to the study of hybrid macaque populations.An introduced group of foreign macaque species in the southernmost of the Bousou peninsula, Chiba, was con-firmed to contain at least rhesus monkeys. From genetic analysis of this study, it was revealed that reciprocal hybridization took place between the introduced and native macaques in the peninsula.Distribution and recent expansion of introduced Taiwanese macaques in Izu-ohsima Island was studied with special interests on geographic distribution of mitochondrial DNA variations. Two haplotypes were distributed ubiquitously and this distribution pattern might result from differential expansion of two maternal lineages after the introduction of their parental population to the island.

本研究以日本猕猴与台湾猕猴在和歌山杂交所产生的猕猴族群为研究对象，探讨其遗传特性。利用细胞核、Y染色体和线粒体DNA标记，发现杂交是通过群体雌性与日本移民雄性杂交产生可育后代而发生的，杂交群体中日本猕猴的相对基因频率迅速增加。通过评估Y染色体特异性标记的地理分布，研究了外源基因通过成年雄性转移从杂交群体中的扩散。没有证据表明基因从引进的种群流向邻近省份的种群。天然耐药相关巨噬细胞蛋白1（NRAMPI）的诊断核标记物是新开发的杂交评估。本文还对AFLP技术在猕猴杂交种群研究中的应用作了初步探讨，证实了在釜山半岛最南端的千叶的一个外来猕猴种群中至少含有恒河猴。本研究的遗传分析显示，引进猕猴与本地猕猴在半岛发生了相互杂交。本研究探讨了引进台湾猕猴在伊豆大岛的分布和最近的扩张，特别关注线粒体DNA变异的地理分布。两种单倍型普遍分布，这种分布模式可能是由于两个母系的差异扩张后，他们的父母人口引进到岛上。

项目成果

Asada Y, Kawamoto Y, Shotake T, Terao K: "Molecular evolution of lgG subclass among nonhuman primates: implication of differences in antigenic determinants among apes."Primates. 43-3. 343-349 (2002)

Asada Y、Kawamoto Y、Shotake T、Terao K：“非人类灵长类动物 IgG 亚类的分子进化：猿类之间抗原决定簇差异的影响。”灵长类动物。

早石周平: "ヤクシマザル(Macaca fuscata yakui)における糞を用いた齢クラス推定とミトコンドアDNA変異の分析"哺乳類科学. 42・2. 161-166 (2002)

Shuhei Hayaishi：“利用屋久岛猕猴（Macaca fuscata yakui）的粪便和线粒体 DNA 变异进行年龄分级的分析”《哺乳动物科学》42・2（2002 年）。

萩原 光: "房総半島におけるニボンザルと外来種の混血に関する研究"霊長類研究. 18・3. 384 (2002)

萩原光：《房总半岛日本猴与外来物种的杂交研究》《灵长类研究》18・3（2002）。

川本 芳: "ニホンザルのミトコンドリア遺伝子変異の地理的分布"霊長類研究. 17巻・2号. 144 (2001)

Yoshi Kawamoto：“日本猕猴线粒体基因突变的地理分布”灵长类动物研究，第 17 卷，第 2.144 期（2001 年）。

泊賢一朗: "東日本におけるニホンザル個体群のタンパク多型解析"霊長類研究. 18・3. 365 (2002)

Kenichiro Tomari：“日本东部日本猕猴种群的蛋白质多态性分析”灵长类研究18・365（2002）。