Studies on genetic changes in interspecific hybridization of primates

本研究以日本猕猴与台湾猕猴在和歌山杂交所产生的猕猴族群为研究对象，探讨其遗传特性。利用细胞核、Y染色体和线粒体DNA标记，揭示了杂交是通过群体雌性与日本移民雄性杂交产生可育后代而发生的，并且在杂交群体中日本猕猴的相对基因频率迅速增加。通过评估Y染色体特异性标记的地理分布，研究了外源基因通过成年雄性转移从杂交群体中的扩散。没有证据表明基因从引进的种群流向邻近省份的种群。天然耐药相关巨噬细胞蛋白1（NRAMPI）的诊断核标记物是新开发的杂交评估。本文还对AFLP技术在猕猴杂交种群研究中的应用作了初步探讨，证实了在釜山半岛最南端的千叶的一个外来猕猴种群中至少含有恒河猴。本研究的遗传分析显示，引进猕猴与本地猕猴在半岛发生了相互杂交。本研究探讨了引进台湾猕猴在伊豆大岛的分布和最近的扩张，特别关注线粒体DNA变异的地理分布。两种单倍型普遍分布，这种分布模式可能是由于两个母系的差异扩张后，他们的父母人口引进到岛上。

A macaque population produced by the hybridization of native Japanese macaques and introduced Taiwanese macaques in Wakayama was studied for its genetic features.. Using nuclear, Y chromosome and mitochondrial DNA markers, it was revealed that the hybridization had occurred through interbreed of group females with immigrant Japanese males by producing fertile offspring and that relative gene frequencies of Japanese macaques increased rapidly in the hybrid population. Dispersal of exotic genes from the hybrid population by adult male transfer was investigated by assessing geographic distribution of Y chromosome specific markers. There was no evidence of gene flow from the introduced population to those in the neighboring provinces. A diagnostic nuclear marker of natural resistance-associated macrophage protein 1 (NRAMPI) was newly developed for the assessment of hybridization. Preliminary results were also obtained for the applicability of amplified fragment length polymorphism (AFLP) to the study of hybrid macaque populations.An introduced group of foreign macaque species in the southernmost of the Bousou peninsula, Chiba, was con-firmed to contain at least rhesus monkeys. From genetic analysis of this study, it was revealed that reciprocal hybridization took place between the introduced and native macaques in the peninsula.Distribution and recent expansion of introduced Taiwanese macaques in Izu-ohsima Island was studied with special interests on geographic distribution of mitochondrial DNA variations. Two haplotypes were distributed ubiquitously and this distribution pattern might result from differential expansion of two maternal lineages after the introduction of their parental population to the island.