Development of microfluidic devices utilizing electroosmotic forces and self-organization of ferromagnetic colloidal particles

(1)对电渗现象进行了理论分析，开发了包括电渗力、电泳力和电流体动力学在内的微流体动力学和微通道设计程序。(2)研究了团簇的生长过程。研究了铁磁性颗粒分散体系的团簇结构和流变特性，得到了以下结果：(1)阐明了交流磁场诱导负粘性效应的机理；(2)阐明了交流磁场频率对颗粒聚集过程的影响；(3)发展了一种利用团簇在旋转磁场中的动力学强化生物化学反应的有效方法。(4)提出了一种利用旋转磁场操纵铁磁性颗粒和顺磁颗粒的新方法。(3)阐明了顺磁颗粒分散体系中团簇的生长过程和团簇结构。我们开发了一种利用激光在硅和石英衬底上制作10-100μm通道等微结构的方法。(5)我们开发了一种激光加工方法来制作三维复杂的微通道，从而有效地混合了微流体。(6)我们制作了一个原型微生物系统，证实了可以测量红细胞的力学性质，并可以准确地计数细胞数量。

We developed basic methodologies and technologies for the design of future micro-bio analytical chips and obtained the following results:(1)We analyzed the electroosmotic phenomena theoretically and developed programmers of both the microfluid dynamics and the microchannels design, in which the electroosmotic forces, electrophoresis and electrofluid dynamics are included.(2)We investigated the cluster growth process, cluster structures and rheological characteristics of ferromagnetic particles' disperse systems and obtained the following results:(a)We clarified the mechanism of the negative viscosity effect induced by ac magnetic fields.(b)We clarified the effect of the frequency of ac magnetic fields on the particles' aggregation processes.(c)We developed an effective method of enhancing bio-chemical reactions utilizing the clusters' dynamics in a rotational magnetic field.(d)We developed a new manipulation method of ferro and paramagnetic particles utilizing a rotational magnetic field.(3)We clarified the cluster growth process and cluster structures in a paramagnetic particles' disperse system, which is subjected to a dc magnetic field.(4)We developed a method of producing microstructures such as 10 -100 μm channels on silicon and quartz substrates using a laser beam.(5)We developed a laser processing method of producing 3-dimensional complex micro channels, thanks to which microfluids are mixed efficiently.(6)We made a prototype micro-bio system and confirmed that the mechanical properties of red blood cells can be measured and that the number of cells can be counted accurately.