Perfect prevention of oxidation of material with biological activity using regeneration reaction of lipid soluble antioxidant with catechin

利用儿茶素脂溶性抗氧化剂的再生反应，完美防止生物活性物质的氧化

• 批准号: 13450323
• 负责人: YONEMOTO Toshikuni
• 金额: $ 9.28万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13450323/
• 关键词: biological activated material; antioxidant; prevention of oxidation; β-carotene; vitamin E; catechin; 脂溶性抗酸化剤; 油水二相系; 速度論モデル; 抗酸化活性; ラジカル捕捉型抗酸化剤; 活性酸素

The purpose of this research is to evaluate the antioxidant activity of catechin and to construct a novel method for preventing the oxidation of desired materials with biological activity in the actual food system using a regeneration reaction of lipid soluble antioxidant with catechin. In 2002, the oxidation experiments of β-carotene, an active oxygen quencher, were performed in an inert n-decane solvent with addition of epigallocatechin gallate (EGCG) or epicatechin gallate (ECG). In both cases, the induction period, in which the β-carotene concentration decreased slightly, was observed and the oxidation was suppressed. The induction period became longer with increasing the ECG concentration, whereas the period had a maximum at a certain EGCG concentration. EGCG containing pirogallol group is known to oxidize itself to produce activated oxygen. Therefore, EGCG was considered to have a progressive activity of the oxidation as well as the antioxidant activity.β-Carotene is commonly dis … More solved in the oil in water emulsion when used as a food additive. Catechin haying amphiphilic property is more easily dissolved in the water phase. The reaction of β-Carotene and lipid-soluble antioxidant, α-tocopherol, with catechin occurs on the oil-water interface. In 2003, the oxidation experiments in the presence of α-tocopherol were performed in the biphasic oil-water system having a definite interfacial area. In the biphasic system, the time for which α-tocopherol remained was longer than that in the single phase of n-decane. Since the β-carotene oxidation was suppressed during a-tocopherol remained in the oil phase, the existence of water phase was effective for the suppression of β-carotene oxidation. α-Tochopherol was consumed by its oxidation as well as the antioxidative protection of β-carotene. The peroxyl radical of α-tocopherol concerned with the chain oxidation was easy to dissolve in the water. This phenomenon seemed to contribute the restrain of α-tocopherol consumption, resulting in the suppression of β-carotene oxidation. A kinetic model was constructed by considering the transfer of the α-tocopherol peroxyl radical into the water phase in addition to the oxidation and antioxidation mechanisms of β-carotene. The model quantitatively described the oxidation behavior of β-carotene in the biphasic system over a wide range of the initial α-tocopherol concentrations. Less

本研究的目的是评价儿茶素的抗氧化活性，并构建一种新的方法，用于防止实际食品系统中具有生物活性的所需物质的氧化，使用脂溶性抗氧化剂与儿茶素的再生反应。2002年，在惰性正癸烷溶剂中，加入表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）或表儿茶素没食子酸酯（epicatechin gallate，ECG），进行了β-胡萝卜素的氧化实验。在这两种情况下，观察到诱导期，其中β-胡萝卜素浓度略有下降，氧化被抑制。诱导期随ECG浓度的增加而延长，但在一定浓度下诱导期达到最大值。已知含有吡罗没食子酸基团的EGCG自身氧化以产生活性氧。因此，EGCG被认为具有渐进的氧化活性以及抗氧化活性。 ...更多信息 当用作食品添加剂时溶解在水包油乳液中。儿茶素具有两亲性，更易溶于水相。β-胡萝卜素和脂溶性抗氧化剂α-生育酚与儿茶素的反应发生在油-水界面上。2003年，在具有一定界面面积的两相油水体系中进行了α-生育酚存在下的氧化实验。在两相体系中，α-生育酚的保留时间比在正癸烷单相体系中长。由于α-生育酚在油相中的存在抑制了β-胡萝卜素的氧化，因此水相的存在对抑制β-胡萝卜素的氧化是有效的。α-生育酚被氧化消耗，β-胡萝卜素的抗氧化保护作用也被消耗。α-生育酚的过氧自由基与链氧化反应有关，易溶于水。这种现象可能有助于抑制α-生育酚的消耗，从而抑制β-胡萝卜素的氧化。在考虑β-胡萝卜素氧化和抗氧化机理的基础上，考虑α-生育酚过氧自由基向水相中的转移，建立了一个动力学模型。该模型定量描述了α-生育酚在较宽初始浓度范围内的氧化行为。少

项目成果

N.Shibasaki-Kitakawa et al.: "Oxidation Kinetics of β-Carotene in Oleic Acid Solvent with Addition of an Antioxidant,α-Tocopherol"Journal of American Oil Chemists' Society. (in printing). (2004)

N. Shibasaki-Kitakawa 等人：“添加抗氧化剂、α-生育酚的油酸溶剂中 β-胡萝卜素的氧化动力学”美国石油化学家协会杂志（2004 年）。

A.Takahashi et al.: "A Rigorous Kinetic Model for β-Carotene Oxidation in the Presence of an Antioxidant, α-Tocopherol"JAOCS. 80. 1241-1247 (2003)

A. Takahashi 等人：“抗氧化剂 α-生育酚存在下 β-胡萝卜素氧化的严格动力学模型”JAOCS 80. 1241-1247 (2003)

A.Takahashi et al.: "A Kinetic Model for Co-oxidation of β-Carotene with oleic Acid"JAOCS. 78. 1203-1207 (2001)

A. Takahashi 等人：“β-胡萝卜素与油酸共氧化的动力学模型”JAOCS 78. 1203-1207 (2001)。

A.Takahashi et al.: "Lipid Oxidation Pathway-Chapter 5 Kinetic Analysis of β-Carotene Oxidation in a Lipid Solvent or Without an Antioxidant"Editor Afaf Kamal-Eldin, AOCS Press. 323(111-137) (2003)

A. Takahashi 等人：“脂质氧化途径 - 第 5 章脂质溶剂或无抗氧化剂中 β-胡萝卜素氧化的动力学分析”编辑 Afaf Kamal-Eldin，AOCS Press 323(111-137) (2003)。

A.Takahashi et al.: "Lipid Oxidation Pathway -Chapter 5 Kinetic Analysis of β-Carotene Oxidation in a Lipid Solvent With or Without an Antioxidant"AOCS Press (Editor Afaf Kamal-Eldin). 323. 111-137 (2003)

A. Takahashi 等人：“脂质氧化途径 - 第 5 章含或不含抗氧化剂的脂质溶剂中 β-胡萝卜素氧化的动力学分析”AOCS Press（Afaf Kamal-Eldin 编辑）323. 111-137 (2003)。