Developement of rotating annular chromatographic reactor for continuous production of pectine oligosaccharide

连续生产果胶寡糖的旋转环形色谱反应器的研制

• 批准号: 07555548
• 负责人: YONEMOTO Toshikuni
• 金额: $ 0.96万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07555548/
• 关键词: chromatographic reactor; immobilized enzyme; oligosaccharide; pectin; 反応クロマト装置; オリゴガラクツロン酸; エンドポリガラクツロナーゼ

In order to develope a rotating annular chromatographic reactor for continuous production of pectin oligosaccharide, it is necessary to clarify the separation performance of the rotating annular chromatography and the kinetics of the consecutive depolymerization using the immobilized enzyme. The hydrolysis experiments of oligosaccharide using both suspended and immobilized enzymes were carried out under various conditions of the initial substrate and enzyme concentrations. Isomaltohexaose, the oligosaccharide of the polymerization degree of 6, was chosen as a substrate, because the mechanism of consecutive depolymerization was comparatively simple and the concentration could be easily analyzed. Endo-dextranase was used as a enzyme. A novel kinetic model for the suspended enzyme system was developed by introducing the selectivity of the enzyme for cleaving each alpha-1,6-glycosidic bond of the substrate. The selectivity coefficient was estimated at 0.411 and almost the same as the exper … More imental values. The model was extended for the immobilized enzyme system by taking into account the intraparticle mass transfer resistance. The model constants were estimated by fitting the extended model with the experimental data obtained under the various conditions.The kinetic constants for the immobilized enzyme system are much larger than those for the suspended enzyme system. The estimated values of effective diffusivities in the support particle were a few order of magnitude smaller than those in aqueous solution. A column reactor packed with the immoblized enzyme was designed and prepared. The experiments of the continuous hydrolysis were carried out using the column reactor under the various conditions of the flow rate, the substrate concentration and the enzyme concentration. A mathematical model for the continuous process was constructed on the basis of the kinetic model for the immobilized enzyme system. The simulated results by the model using the estimated values of the model constants in the immobilized enzyme system were in good agreement with the experimental results. Less

为了开发用于连续生产果胶低聚糖的旋转环状色谱反应器，有必要阐明旋转环状色谱的分离性能和使用固定化酶的连续解聚动力学。在不同的初始底物浓度和酶浓度条件下，进行了悬浮酶和固定化酶水解低聚糖的实验。选择聚合度为6的低聚糖异麦芽六糖作为底物，因为其连续解聚机理比较简单，且浓度易于分析。内切葡聚糖酶用作酶。通过引入酶对底物上每个α-1，6-糖苷键的选择性，建立了一个新的悬浮酶体系动力学模型。选择性系数为0.411，与实验值基本一致 ...更多信息 基本价值观通过考虑颗粒内传质阻力，将该模型推广到固定化酶体系。通过对不同条件下实验数据的拟合，得到了模型常数，固定化酶体系的动力学常数远大于悬浮酶体系的动力学常数。在载体颗粒中的有效扩散系数的估计值比在水溶液中的小几个数量级。设计并制备了固定化酶填充柱反应器。在不同的流速、底物浓度和酶浓度条件下，采用塔式反应器进行连续水解实验。在固定化酶系统动力学模型的基础上，建立了连续过程的数学模型。利用固定化酶系统模型常数的估算值，对固定化酶系统进行模拟，模拟结果与实验结果吻合较好。少

项目成果

N.Shibasaki-Kitakawa et al.: "Kinetic Model for Oligosaccharide Hydrolysis using Suspended and Immobilized Enzymes." Biochemical Engineering Journal. In printing.

N.Shibasaki-Kitakawa 等人：“使用悬浮和固定酶进行低聚糖水解的动力学模型。”

N.Shibadski-Kitazawa et al.: "Kinetic Model Oligosaccharide Hydrolysis using Suspended and Immobilized Enzymes" Biochemical Engineering Journal. (in priting).

N.Shibadski-Kitazawa 等人：“使用悬浮和固定酶水解低聚糖的动力学模型”生物化学工程杂志。