Promotion of growth rate by improving hormonal and metabolic functions by rumen-bypass starch in suckling calves

通过过瘤胃淀粉改善乳牛的激素和代谢功能来促进生长速度

• 批准号: 13460123
• 负责人: OBARA Yoshiaki
• 金额: $ 7.81万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460123/
• 关键词: Suckling calves; Suckling kids; Rumen-bypass starch; Glucose metabolism; Nitrogen metabolism; Endocrine secretion; SGLT-1; Na^+グルコーストランスポーター(SGLT-1); 新生子牛; バイパス澱粉飼料; 同位元素希釈法

The aim of this study was to investigate the kinetics of glucose and nitrogen metabolism when rumen-bypass starch particles were given to suckling calves and kids. Furthermore, we also analyzed the effects on the expression of sodium-dependent glucose transporter (SGLT-1) in the gastrointestinal (GI) tract epithelium. Twelve animals were used for each animal species. Rumen-bypass particles were given to the animals at the age of 6 to 13 weeks. Tissue samples were taken at the age of around 3 and 13 weeks. Glucose and nitrogen metabolism was measured by the stable isotope dilution method with ^2H, ^<13>C-glucose and ^<15>N, ^<13>C-urea, respectively. The plasma concentration of insulin, GH and insulin-like growth factor-I (IGF-I) was measured by radioimmunoassay. The expression of SGLT-1 mRNA was analyzed by the semi-quantitative RT-PCR method. The metabolism of glucose and nitrogen, and plasma concentration of metabolic hormones changed around weaning. In animals fed rumen-bypass particles, plasma concentrations of amino-N and insulin tended to decrease, but other parameters did not change significantly. Although SGLT-1 mRNA was confirmed to express commonly along the suckling kid GI tract with marked expression in the epithelium of duodenum, jejunum and ileum, it was substantially reduced after weaning (at the age of 13 weeks old). However, after weaning, there was no significant difference between control group and the particle-fed group.

本试验旨在研究过瘤胃淀粉颗粒对哺乳犊牛和羔羊糖氮代谢的影响。此外，我们还分析了对钠依赖性葡萄糖转运蛋白（SGLT-1）在胃肠道（GI）上皮中表达的影响。每种动物种属使用12只动物。在6至13周龄时给予动物过瘤胃颗粒。在约3周龄和13周龄时采集组织样品。葡萄糖和氮代谢分别用^2 H，^<13>C-葡萄糖和^<15>N，^<13>C-尿素稳定同位素稀释法测定。用放射免疫法测定血浆胰岛素、生长激素和胰岛素样生长因子-I（IGF-I）浓度。半定量RT-PCR法检测SGLT-1 mRNA的表达。断奶前后，糖、氮代谢及血浆代谢激素水平发生变化。在饲喂过瘤胃颗粒的动物中，血浆氨基-N和胰岛素浓度倾向于降低，但其他参数没有显着变化。虽然SGLT-1 mRNA被证实通常沿着消化道表达，在十二指肠、空肠和回肠上皮中显著表达，但在断奶后（13周龄）其显著降低。而断奶后，对照组和颗粒饲料组之间无显著差异。

项目成果

Y.Obara: "Effects of aging and feeding condition on the growth-related Physiological functions in suckling calves"Proc. Jpn. Soc. Anim. Nutr. Metab.. Vol.45. 65-74 (2001)

Y.Obara：“衰老和饲养条件对乳牛生长相关生理功能的影响”Proc。

S.Yonekura: "Effects of aging and weaning on mRNA expression of leptin and CCK receptors in the calf rumen and abomasum"Domestic Animal Endocrinology. Vol.22. 25-35 (2002)

S.Yonekura：“衰老和断奶对小牛瘤胃和真胃中瘦素和 CCK 受体 mRNA 表达的影响”国内动物内分泌学。

Kitade K. et al.: "Effects of nutritional conditions around weaning on carbonic anhydrase activity in the parotid gland and ruminal and abomasal epitheria of Holstein calves"J. Comp. Physiol.. 172B. 379-385 (2003)

Kitade K. 等人：“断奶前后营养条件对荷斯坦犊牛腮腺、瘤胃和真胃上皮中碳酸酐酶活性的影响”J。

Obara Y.: "Effects of aging and feeding condition on the growth-related Physiological functions in suckling calves"Proc. Jpn. Soc. Anim. Nutr. Metab.. 45. 65-74 (2001)

Obara Y.：“衰老和饲养条件对乳牛生长相关生理功能的影响”Proc。

小原 嘉昭: "哺乳子牛の発育に伴う生理機能の変動に及ぼす加齢と飼養形態の影響"栄養生理研究会報. 第45巻・第2号. 65-74 (2001)

Yoshiaki Ohara：“衰老和喂养方式对乳牛生长相关生理功能变化的影响”《营养生理学研究杂志》，第 45 卷，第 2. 65-74 期（2001 年）。