Study of Baylisascaris procyonis larva migrans : Pathological and parasitological analysis and development of serological diagnosis.

Baylisascaris procyonis 幼虫移行性研究：病理学和寄生虫学分析以及血清学诊断的发展。

• 批准号: 13460137
• 负责人: FURUOKA Hidefumi
• 金额: $ 8.32万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460137/
• 关键词: Baylisascaria procyonis; Larva migrans; Serodiagnosis; Pathology; Parasitology; 病理; 寄生虫; 血清診断; 感染実験; 感染モデル; 血清疫学

Larva migrans caused by the common raccoon ascarid, Baylisascaris procyonis, is a zoonotic disease of critical importance in North America. We encountered the first proven outbreak of this disease in Japan in domestic rabbits in a small wildlife park. In this park, raccoons had been kept for 9 years, and one raccoon was donated to the park by a pet owner 8 weeks prior to the occurrence of an outbreak in rabbits. Circumstantial evidence indicates that the donated raccoon is the likely source of this outbreak. Also, we investigated the details of the central nervous lesion in the rabbits affected with B.procyonis larva migrans in Japan. In this study, we demonstrated ascarid larvae in only eight out of 23 animals diagnosed as B.procyonis larva migrans. Since it is not always possible to detect the larvae, the possibility of B.procyonis larva migrans must be given serious consideration to the characteristic lesions. Also, B.procyonis causes a severe retinal lesion in humans. The lesion is termed as diffuse unilateral subacute neuroretinitis (DUSN). To understand the pathogenesis of B.procyonis in gerbils, we inoculated the embryonated eggs of B.procyonis into Mongolian gerbils, and monitored their fundi with an ophthalmoscope. Histopathological examination revealed perivasculitis in the optic disk region, inflammatory proliferation of the pigment cells, and vitreitis in most cases. These findings were similar to those in human cases of DUSN, suggesting that gerbils might be a useful model for understanding the pathogenesis of B.procyonis infection in humans.

幼虫移行症是由常见的浣熊蛔虫引起的一种重要的动物传染病。我们在日本的一个小型野生动物园的家兔中首次发现了这种疾病的爆发。在这个公园里，浣熊已经被饲养了9年，其中一只浣熊是在兔子爆发前8周由宠物主人捐赠给公园的。间接证据表明，捐赠的浣熊可能是这次疫情的源头。此外，我们还研究了日本感染B.procyonis幼虫移行症的家兔中枢神经病变的详细情况。在这项研究中，我们证明了蛔虫幼虫在23只动物诊断为B.procyonis幼虫移行症。由于并不总是能够检测到幼虫，因此必须认真考虑B.procyonis幼虫移行的可能性。此外，B.procyonis在人类中引起严重的视网膜病变。这种病变被称为弥漫性单侧亚急性神经视网膜炎（DUSN）。为了解B.procyonis在沙鼠体内的致病机制，我们将B.procyonis的含胚卵接种于蒙古沙鼠体内，并用检眼镜监测其胃底。组织学检查显示视盘区血管周围炎，色素细胞炎性增生，大多数病例有玻璃体炎。这些发现与人类DUSN病例相似，表明沙鼠可能是了解人类感染B.procyonis发病机制的有用模型。

项目成果

Nobuaki Akao: "Diffuse retinochoroiditis due to Baylisascaris procyonis in Monglian gerbils."J Parasitol. 89(1). 174-175 (2003)

Nobuaki Akao：“蒙古沙鼠中由 Baylisascaris procyonis 引起的弥漫性视网膜脉络膜炎。”J Parasitol。

Nobuaki Akao: "Diffuse retinochoroiditis due to Baylisascaris procyonis in Monglian gerbils."J Parasitol.. 89(1). 174-175 (2003)

Nobuaki Akao：“蒙古沙鼠中由 Baylisascaris procyonis 引起的弥漫性视网膜脉络膜炎。”J Parasitol.. 89(1)。

Hiroshi Sato: "First outbreak of Baylisascaris procyonis larva migrans in rabbits in Japan."Parasitol Int. 51(1). 105-108 (2002)

Hiroshi Sato：“日本兔子中首次爆发贝利斯蛔虫幼虫移行症。”Parasitol Int.

Hiroshi Sato: "First outbreak of Baylisascaris procyonis larva migrans in rabbits in Japan."Parasitol Int.. 51(1). 105-108 (2002)

Hiroshi Sato：“日本兔子中首次爆发贝里斯蛔虫幼虫移行症。”Parasitol Int.. 51(1)。

Hiroshi Sato: "Epidemiological aspects of the first outbreak of Baylisascaris procyonis larva migrans in rabbits in Japan."J Vet Med Sci. 65(4). 453-457 (2003)

Hiroshi Sato：“日本兔子中首次爆发的贝里斯蛔虫幼虫移行症的流行病学方面。”J Vet Med Sci。