IMMUNOCHEMICAL AND PROTEINOLOGICAL STUDY IN THE HEREDITARY MYOPATHY OF THE DIAPHRAGMATIC MUSCLES IN CATTLE

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In several cases of the cattle which were submitted to the necropsy due to the various diseases, numerous core-like structures without accompanying muscle degeneration have been found in the diaphragmatic and intercostal muscles, and myocardium. Of the 213 heads studied from 8 months to 15 years old, 49 cases were affected this disorder. Addition to these pathological findings which showed a typical the core-like structures seen in the hereditary myopathy of the diaphragmatic muscles, the 49 cases were deribed from the same family line in the cases of this myopathy. These observations suggested that the 49 cases might have the potency of this myopathy.In order to investigate the pathogenesis of this myopathy, antibodies for the both diseased and normal muscle tissue, respectively, were prepared for the immunoblotting analysis. The immunoblots of homogenates of the diseased muscles demonstrated the presence of two specific bands (approximately 55 and 65 kDa) and decreased level of immunoreactivity (about 85 kDa). Although this study failed to reach the identification of these abnormal proteins, they seemed to play some part in the pathogenesis of this myopathy.Since the late 1990s, we encountered a considerable number of cases of the dilated cardiomyopathy. By histology, the myocardium revealed hypertrophy and vacuolation of the cardiac muscle fibers and severe fibrosis. Pedigree analysis of affected cattle indicated an autosomal recessive mode of inheritance. The family line of this cardiomyopathy overlapped with the one of the hereditary myopathy of the diaphragmatic muscles in Holstein-Friesian cattle, although those disorders exhibit a varying pathology. The genetic basis may support those different phenotypes appearing from one family tree.