Structure and function of ryanodine receptor

兰尼定受体的结构和功能

• 批准号: 13470028
• 负责人: TAKESHIMA Hiroshi
• 金额: $ 8.06万
• 依托单位: Tokoku University (2002)Kurume University (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470028/
• 关键词: ryanodine receptor; junctophilin; intracellular calcium ions; calcium release; junctional membrane; Ca^<2+>ストア; 興奮収縮連関; Ca^<2+>チャネル; 三つ組構造

In excitable cell types, the signal conversion from depolarization to intracellular Ca2+ rise requires functional coupling between cell-surface Ca2+ channels and intracellular Ca2+ release channels. In this project, our research group focused on (i) the physiological regulation of ryanodine receptor subtype channels, (ii) the physiological roles of junctophilin subtypes (junctophilins contribute to the formation of junctional membrane complexes observed in excjtable cells), (iii) identification of new membrane proteins taking part in functions of intracellular Ca2+ stores. Major findings in the studies are contribution of ryanodine receptors to learning and memory in the central neurvous system, contribution of junctophilins to motorcoordination in certain neurons, and identification of mitsugumin53 as a novel membrane protein in striated muscles.

在可兴奋的细胞类型中，从去极化到细胞内Ca2+上升的信号转换需要细胞表面Ca2+通道和细胞内Ca2+释放通道之间的功能耦合。在这个项目中，我们的研究小组集中在(i) ryanodine受体亚型通道的生理调节，（ii）结亲蛋白亚型的生理作用（结亲蛋白有助于在可兴奋细胞中观察到的连接膜复合物的形成），（iii）鉴定参与细胞内Ca2+储存功能的新膜蛋白。本研究的主要发现包括：在中枢神经系统中，ryanodine受体参与学习和记忆；在某些神经元中，junctophilins参与运动协调；在横纹肌中发现了一种新的膜蛋白mitsugumin53。

项目成果

Komazaki et al.: "Deficiency of triad formation in developing skeletal muscle cells lacking junctophilin type 1"FEBS Lett.. 524. 225-229 (2002)

Komazaki 等人：“缺乏 1 型连接蛋白的发育骨骼肌细胞中三联体形成的缺陷”FEBS Lett.. 524. 225-229 (2002)

Komazaki 他3名: "Deficiency of triad formation in developing skeletal muscle cells lacking junctophilin type 1."FEBS Lett.. 524. 225-229 (2002)

Komazaki 和其他 3 人：“缺乏 1 型连接蛋白的发育骨骼肌细胞中三联体形成的缺陷。”FEBS Lett.. 524. 225-229 (2002)

Higgins et al.: "A combined pharmacological and genetic approach to investigate the role of orphaninFQ in learning and memory"Eur. J. Neurosci.. 15. 911-922 (2002)

Higgins 等人：“采用药理学和遗传学相结合的方法来研究孤儿蛋白 FQ 在学习和记忆中的作用”Eur。

Uehara et al.: "Store-operated Ca2+ entry irrelevant to Ca2+ release channel and junctional membrane complex in heart muscle cells"Cell Calcium. 31. 89-96 (2002)

Uehara 等人：“与心肌细胞中 Ca2 释放通道和连接膜复合体无关的存储操作 Ca2 进入”细胞钙。

Pan et al.: "Dysfunction of store-operated Ca2+ channel in muscle cells lacking mg29 gene"Nature Cell Biol.. 4. 379-383 (2002)

Pan 等人：“缺乏 mg29 基因的肌肉细胞中钙池操纵的 Ca2 通道功能障碍”Nature Cell Biol.. 4. 379-383 (2002)