Roles of novel intracellular ion channels

新型细胞内离子通道的作用

• 批准号: 20249004
• 负责人: TAKESHIMA Hiroshi
• 金额: $ 28.45万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249004/
• 关键词: TRICチャネル; ミツグミン23; 小胞体; 細胞内Ca^<2+>ストア; リアノジン受容体; 細胞内カルシウムストア; IP3受容体; カルミン; Ca2+放出; ミツグミン53

Our group recently identified the TRIC channel and mitsugumin 23 (MG23) as novel intracellular ion channels, and focused on their structure-function relationships and physiological roles in this study. We defined electrophysiological characteristics of TRIC channel subtypes in single channel measurements and their essential physiological functions in alveolar epithelial and skeletal muscle cells using knockout mice. Moreover, we also clarified the three-dimentional volume and cation-specific channel feature of MG23 in single-particle imaging and single channel monitoring, respectively.

本课题组最近发现TRIC通道和Mitsugumin 23（MG 23）是新的细胞内离子通道，并对其结构功能关系和生理作用进行了研究。我们定义了TRIC通道亚型在单通道测量中的电生理特征及其在使用敲除小鼠的肺泡上皮细胞和骨骼肌细胞中的基本生理功能。此外，我们还阐明了MG 23在单粒子成像和单通道监测中的三维体积和阳离子特异性通道特征。

项目成果

Sarcalumenin alleviates stress-induced cardiac dysfunction by improving Ca2+ handling of the sarcoplasmic reticulum

• DOI: 10.1093/cvr/cvm019
• 发表时间: 2008-01-15
• 期刊: CARDIOVASCULAR RESEARCH
• 影响因子: 10.8
• 作者: Shimura, Miei;Minamisawa, Susumu;Ishikawa, Yoshihiro
• 通讯作者: Ishikawa, Yoshihiro

Single-channel characterization of the rabbit recombinant RyR2 reveals a novel inactivation property of physiological concentrations of ATP

兔重组 RyR2 的单通道表征揭示了生理浓度 ATP 的新失活特性

• 发表时间: 2008
• 期刊: J. Membr. Biol 65
• 作者: Stewart R;Song L;Carter SM;Sigalas C. Zaccai NR;et.al.
• 通讯作者: et.al.

Mitsugumin 53-mediated maintenance of K+ currents in cardiac myocytes.

• DOI: 10.4161/chan.3.1.7571
• 发表时间: 2009-01
• 期刊: Channels (Austin, Tex.)
• 作者: Masumiya H;Asaumi Y;Nishi M;Minamisawa S;Adachi-Akahane S;Yoshida M;Kangawa K;Ito K;Kagaya Y;Yanagisawa T;Yamazaki T;Ma J;Takeshima H
• 通讯作者: Takeshima H

Facilitation of DNA damage-induced apoptosis by endoplasmic reticulum protein mitsugumin23.

• DOI: 10.1016/j.bbrc.2010.01.013
• 发表时间: 2010-02
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: T. Yamazaki;Nozomi Sasaki;M. Nishi;H. Takeshima

Imparied Orail-mediateded resting Ca^<2+> entry reduces the cytosolic [Ca^<2+>] and SR Ca^<2+> loading in quitescent junctophilinl knockout myotubes.

受损的Orail介导的静息Ca ^ 2 进入减少了静止嗜连接蛋白敲除肌管中的胞质[Ca ^ 2 ]和SR Ca ^ 2 负载。

• 发表时间: 2010
• 期刊: J.Biol.Chem. 285
• 作者: Li;H.;Ding;X.;Lopez;J.R.;Takeshima;H.;Ma;J.;Allen;P.D.;Eltit;J.M.
• 通讯作者: J.M.