A Molecular Study on Activation of Circulating Leukocytes in Metal-Induced Lung

金属诱导肺循环白细胞活化的分子研究

• 批准号: 13470080
• 负责人: KUSAKA Yukinori
• 金额: $ 2.18万
• 依托单位: University of Fukui (2004)福井医科大学 (2001-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470080/
• 关键词: Metal; ultrafine powder; nickel; cobalt; leukocyte; interleukin; tumor necrosis factor; monocyte; 超微細金属粒子; ヒト白血球; サイトカイン; 鉄; 金属超微細粒子; TIF-α; NF-κB; ELISA; in-vitro実験系; 活性化

Activation of leukocytes was studied in leukocytes from ultrafine-nickel instilled rat and in leukocytes from healthy volunteers. In the former, at seven days after rats were instilled with Ut-Ni, peripheral leukocytes were recovered and cultured with Ut-Ni. NO and tumor necrosis factor-alpha were highly released from leukocytes. In the latter, peripheral leukocytes recovered from healthy volunteers and separated into monocyte and polymorph nuclear subsets. The two subsets were cultured with Ut-Ni, Ut-Co, and Ut-Fe. Ut-Ni caused release interleukin 6 and 8, and TNF-alpha from the monocyte subset most highly among the three metals. On the other hand, Ut-Fe caused IL-1 most highly from the mononuclear subset. Ut-Ni did no release of cytokines from the polumorphonuclear subset. Taken together, respiratory exposure to Ut-Ni was suggested to cause activation of peripheral leukocytes. In human, if exposure to Ut-Ni of peripheral monocytes occur, activation may follow.

研究了白细胞的激活在超细核细胞中的白细胞和健康志愿者的白细胞中的活化。在前者中，在用UT-NI灌输大鼠后的7天后，回收了周围白细胞并用UT-NI培养。 NO和肿瘤坏死因子-Alpha从白细胞高度释放。在后者中，从健康的志愿者中回收的周围白细胞，分为单核细胞和多形核亚群。这两个子集用UT-NI，UT-CO和UT-FE培养。 UT-NI引起了白介素6和8的释放，在三分属中，单核细胞子集中的TNF-Alpha最高。另一方面，UT-FE从单核子集引起的IL-1最高。 UT-NI没有从果实核子集中释放细胞因子。综上所述，建议呼吸道暴露于UT-NI，以引起周围白细胞的激活。在人类中，如果发生暴露于周围单核细胞的UT-NI，则可能会激活。

项目成果

Cytekine pleases from Human Leukocyte Subsets Cultured in Vitro with Nano-Praticles of Iron Family

使用铁族纳米粒子体外培养的人类白细胞亚群中的细胞因子

• 发表时间: 2005
• 期刊: Proceeding of International Conference on Occupational Environmental Respiratory Diseases, Beijing (in press)
• 作者: M.Muraoaka;K.Sato;N.Suganuma;Y.Kusaka
• 通讯作者: Y.Kusaka

菅沼成文, 日下幸則 他: "「呼吸器学」のうち"じん肺症(石綿肺、珪肺)""メジカルビュー杜. 3-372 (2004)

Shigefumi Suganuma、Yukinori Kusaka 等人：《呼吸病学》中的“肺尘埃沉着病（石棉肺、矽肺）”，Medical View Mori 3-372 (2004)。

Occupational Asthma

• DOI: 10.1081/jas-120000801
• 发表时间: 2002-01
• 期刊: Journal of Asthma
• 影响因子: 1.9
• 作者: A. Draper

The Study for searching allergy material from exposure to metal in hard Metal factory by instrumental neutron activation analysis

仪器中子活化分析寻找硬质合金工厂金属接触过敏物质的研究

• 发表时间: 2003
• 期刊: Biomed Res Trace Elements. 14(4)
• 作者: S.Omori;A.Nakajima;Y.Endo;Y.kusaka
• 通讯作者: Y.kusaka

Cytokine releases from human leukocyte subsets cultured in vitro with nano-particles of iron family metals.

用铁族金属纳米颗粒体外培养的人类白细胞亚群释放细胞因子。

• 发表时间: 2005
• 期刊: Proceeding of 10^<th> International conference on occupational respiratory diseases(In : D.Li, et al.(eds)) (in press)
• 作者: Muaroka;M.;Suganuma;N.;Satoh;K.;Kusaka
• 通讯作者: Kusaka