Clarification of transmission mechanism of urinary sensation in pontine micturition center and central effect of drugs for voiding dysfunction.

我们进行了电生理研究，以阐明脑桥排尿中枢和排尿之间的关系。将导管插入膀胱并注射生理盐水，直至诱导自发收缩。将玻璃移液管微电极插入小脑。单个神经活动记录在中桥脑被盖，包括巴林顿的核，同时其精确的时间关系到膀胱收缩进行了观察。三种类型的神经元被区分开来：那些只在收缩开始前放电的神经元（E1），那些在收缩前不久开始放电并在收缩期间维持放电的神经元（E2），以及那些在收缩期间放电被强烈抑制的神经元（I）。结果表明，引起膀胱收缩的直接神经信号可能来自巴灵顿核。为了探讨排尿功能障碍药物对排尿中枢的影响，在记录E1和E2神经元的同时，静脉注射抗胆碱能药物（盐酸奥昔布宁）和三环类抗抑郁药（盐酸氯丙咪嗪）。随着奥昔布宁的管理，神经放电增加，按照膀胱收缩的抑制。另一方面，神经放电减少与氯丙咪嗪的管理。从这些结果看来，奥昔布宁和氯丙咪嗪具有相似的外周效应和不同的中枢效应。为了阐明去氨加压素对排尿中枢的作用，将其通过静脉和脑室给药。随着给药，观察到神经元，其中放电根据膀胱收缩的抑制而改变。这些结果提示去氨加压素中枢作用的可能性

We performed electrophysiological studies in order to clarify the relationship between the pontine micturition center and micturition. A catheter was inserted into the bladder and saline was injected until spontaneous contraction was induced. A glass pipette microelectrode was inserted through the cerebellum. Single neural activity was recorded in the mesopontine tegmentum including the Barrington's nucleus and simultaneously its precise timing in relation to bladder contraction was observed. Three types of neuron were distinguished ; those which fired only prior to the start of contraction (E1), those whose firing started shortly prior to and was maintained during contraction (E2), and those whose firing, was strongly suppressed during contraction (I). The results showed that direct neural signals to induce bladder contraction might arise from the Barrington's nucleus. The neurons were classified into three types with different functional roles.In order to clarify the effects of drugs for voiding dysfunction on the micturition center, an anticholinergic drug (oxybutynin hydrochloride) and a tricyclic antidepressant (clomipramine hydrochloride) were administered intravenously during the recording of E1 and E2 neurons. With the administration of oxybutynin, neural firing increased in accordance with the suppression of bladder contraction. On the other hand, neural firing decreased with the administration of clomipramine. From these results, it seemed that oxbutynin and clomipramine had similar peripheral effects and different central effects. In order to clarify the effects of desmopressin on the micturition center, it was administered intravenously and intraventriculary. With the administration, neurons were observed in which the firing changed in accordance with the suppression of bladder contraction. From these results, the possibility of the central effect of desmopressin was suggested