Clarification of transmission mechanism of urinary sensation in pontine micturition center and central effect of drugs for voiding dysfunction.

阐明脑桥排尿中枢尿感传导机制及药物治疗排尿功能障碍的中枢作用。

• 批准号: 13470340
• 负责人: KAWAUCHI Akihiro
• 金额: $ 6.53万
• 依托单位: Kyoto Prefectural University of Medicine (KPUM)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470340/
• 关键词: Poms; Micturition center; Barrington's nuclens; Neurons; Firing; oxybutynin; Clomipramine; Desmopressin

We performed electrophysiological studies in order to clarify the relationship between the pontine micturition center and micturition. A catheter was inserted into the bladder and saline was injected until spontaneous contraction was induced. A glass pipette microelectrode was inserted through the cerebellum. Single neural activity was recorded in the mesopontine tegmentum including the Barrington's nucleus and simultaneously its precise timing in relation to bladder contraction was observed. Three types of neuron were distinguished ; those which fired only prior to the start of contraction (E1), those whose firing started shortly prior to and was maintained during contraction (E2), and those whose firing, was strongly suppressed during contraction (I). The results showed that direct neural signals to induce bladder contraction might arise from the Barrington's nucleus. The neurons were classified into three types with different functional roles.In order to clarify the effects of drugs for voiding dysfunction on the micturition center, an anticholinergic drug (oxybutynin hydrochloride) and a tricyclic antidepressant (clomipramine hydrochloride) were administered intravenously during the recording of E1 and E2 neurons. With the administration of oxybutynin, neural firing increased in accordance with the suppression of bladder contraction. On the other hand, neural firing decreased with the administration of clomipramine. From these results, it seemed that oxbutynin and clomipramine had similar peripheral effects and different central effects. In order to clarify the effects of desmopressin on the micturition center, it was administered intravenously and intraventriculary. With the administration, neurons were observed in which the firing changed in accordance with the suppression of bladder contraction. From these results, the possibility of the central effect of desmopressin was suggested

我们进行了电生理学研究，以阐明脑桥排尿中枢与排尿之间的关系。将导管插入膀胱并注射盐水直至诱导自发收缩。将玻璃移液管微电极插入小脑。在包括巴灵顿核在内的中脑桥被盖中记录单个神经活动，同时观察其与膀胱收缩相关的精确时间。区分了三种类型的神经元；那些只在收缩开始之前放电的人（E1），那些在收缩之前不久开始放电并在收缩期间维持的人（E2），以及那些在收缩期间放电被强烈抑制的人（I）。结果表明，诱导膀胱收缩的直接神经信号可能来自巴林顿核。将神经元分为三种类型，具有不同的功能作用。为了明确排尿功能障碍药物对排尿中枢的影响，在记录E1和E2神经元时，静脉注射抗胆碱能药物（盐酸奥昔布宁）和三环类抗抑郁药（盐酸氯米帕明）。服用奥昔布宁后，神经放电随着膀胱收缩的抑制而增加。另一方面，服用氯米帕明后神经放电减少。从这些结果来看，奥昔布宁和氯米帕明似乎具有相似的外周作用和不同的中枢作用。为明确去氨加压素对排尿中枢的影响，采用静脉注射和心室内注射给药。通过给药，观察到神经元的放电随着膀胱收缩的抑制而变化。从这些结果中，提出了去氨加压素的中枢作用的可能性

项目成果

Kawauchi A., et al.: "Relationships among nocturnal urinary volume, bladder capacity and nocturia with and without load in nonenuretic children"Urology. 59. 433-437 (2002)

Kawauchi A. 等人：“非遗尿症儿童夜间尿量、膀胱容量和有负荷或无负荷的夜尿之间的关系”泌尿学。

Tanaka Y.: "Effects of the acupuncture to the sacral segment on the bladder activity and EEG"Psychiatry and Clinical Neurosciences. (in press). (2002)

Tanaka Y.：“骶段针灸对膀胱活动和脑电图的影响”精神病学和临床神经科学。

Tanaka Y., et al.: "Firing of micturition center neurons in the rat mesopontine tegmentum during urinary bladder contraction"Brain Res. 965. 146-154 (2003)

Tanaka Y.等人：“膀胱收缩期间大鼠中桥脑被盖中排尿中枢神经元的放电”Brain Res。

Kawauchi A., et al.: "Relationships among nocturnal urinary volume, bladder capacity, and nocturia with and without water load in nonenuretic children"UROLOGY. 59. 433-437 (2002)

Kawauchi A. 等人：“非遗尿症儿童夜间尿量、膀胱容量和有水负荷和无水负荷的夜尿之间的关系”泌尿学。

Kawauchi A., et al.: "Bladder capacity at the time of enuresis"Urology. 61(in press). (2003)

Kawauchi A. 等人：“遗尿时的膀胱容量”泌尿学。