权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Effects of acupuncture to the sacral segment on the brainstem micturition center in rats

针刺骶段对大鼠脑干排尿中枢的影响

基本信息

批准号：
12671551
负责人：
UKIMURA Osamu
金额：
$ 2.24万
依托单位：
Kyoto PrefecturalUniversity of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671551/
关键词：
acupuncture micturition center overactive bladder electrophyshiology incontinence bladder neuron ニュウロン鍼

项目摘要

Acupuncture to the sacral segment clinically improves urinary incontinence and increases bladder capacity in the patients of overactive bladder. To make clear this mechanism we examined effects of acupuncture to the sacral segment on the bladder activity and the neurons that are considered to be involved micturition. Experiments were performed in adult male rats under urethane anesthesia. Bladder pressure was recorded through a polyethylene catheter inserted from the dome into the urinary bladder, and saline was injected to the bladder from the catheter until it induced spontaneous contraction. Single neuronal activity was recorded through a glass pipette microelectrode in and around the Barrington's nucleus. The electrode was angled posteriorly by 30 ℃ and lowered through the cerebellum. We searched neurons that fired with relation to the bladder contraction. Three types of neurons were recorded. First was neuron almost silent during quiescent period and started to fire period to the bladder contraction. Second was those fired in close relation with bladder contraction. The third was those active during bladder relaxation and became inactive during contraction. When the neuronal activity accompanying spontaneous bladder contraction was recorded, acupuncture was applied. An acupuncture needle (stainless steel, outer diameter ; 0.3 mm) was positioned at the periosteum of sacral segment, and was rotated manually at a speed of about 3 turn/sec for 1 min.During the bladder contraction was suppressed by the acupuncture, activity of all three types of neurons was suppressed. These results suggest that the acupuncture produced suppression of activity of micturition center neurons, Which in turn caused suppression of bladder activity.

针刺骶段可改善膀胱过度活动症患者的尿失禁，增加膀胱容量。为了弄清这一机制，我们研究了针刺骶段对膀胱活动和被认为涉及排尿的神经元的影响。实验在成年雄性大鼠乌拉坦麻醉下进行。通过从穹隆插入膀胱的聚乙烯导管记录膀胱压力，并从导管向膀胱注入生理盐水，直到引起自发收缩。通过玻璃吸管微电极记录巴林顿核内及其周围的单个神经元活动。电极向后倾斜30℃，通过小脑下降。我们搜索了与膀胱收缩有关的神经元。记录到三种类型的神经元。首先是静止期的神经元几乎没有反应，而在膀胱收缩时则开始放电。其次是那些与膀胱收缩密切相关的解雇。第三种是那些在膀胱松弛时活跃，在收缩时变得不活跃的人。记录伴随自发性膀胱收缩的神经元活动时，针刺。将针刺针(不锈钢，外径0.3 mm)放置在骶段骨膜上，以3圈/秒左右的速度手动旋转1min，在针刺抑制膀胱收缩的同时，三种神经元的活动均受到抑制。这些结果提示，针刺可抑制排尿中枢神经元的活动，进而抑制膀胱的活动。