Studies on leukocyte-endothelial interactions in the pathogenesis of diabetic retinopathy

通过体外和体内实验研究白细胞与视网膜血管内皮细胞的相互作用在糖尿病视网膜病变发病机制中的意义。培养人视网膜内皮细胞(HREC)，定量检测中性粒细胞与HREC的黏附能力。胰岛素以剂量依赖的方式显著增加中性粒细胞与HREC的黏附。加入胰岛素可上调HREC ICAM-1的表达。体内研究是针对链脲佐菌素诱导的糖尿病大鼠进行的。用吖啶橙数字荧光照相技术研究视网膜微循环中白细胞的动态变化。与非糖尿病对照组大鼠相比，糖尿病大鼠表现出视网膜白质停滞(视网膜毛细血管内白细胞堆积)。格列齐特和盐酸吡格列酮能有效减少糖尿病大鼠视网膜中白细胞的蓄积。

Significance of interactions between leukocytes and retinal vascular endothelial cells in the pathogenesis of diabetic retinopathy was studied by both in vitro and in vivo. Human retinal endothelial cells (HREC) were cultured and the adhesion of neutrophils to the HREC was quantitated. Insulin significantly increased the adhesion of neutrophils to HREC in the dose dependent manner. ICAM-1 of HREC was upregulated by addition of insulin to the cultured medium. In vivo studies were carried out suing streptozotocin-induced diabetic rats. Acridine orange digital fluorography was performed to study leukocyte dynamics in the retinal microcirculation. Diabetic rats showed retinal leukostasis (accumulation of leukocytes in the retinal capillaries) as compared to the non-diabetic control animals. Gliclzide and pioglitazone hydrochloride effectively decreased the number of accumulated leukocyte in the retina of diabetic rats.