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Experimental studies on application of anti-adhesion therapy for retinal microcirculatory disturbances

抗粘连治疗视网膜微循环障碍的实验研究

基本信息

批准号：
16390501
负责人：
OGURA Yuichiro
金额：
$ 8.7万
依托单位：
NAGOYA CITY UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Leukocyte adhesion to the human retinal vascular endothelial cells was studied in vitro. High glucose concentration and insulin in the culture medium increased the adhesion of neutrophils to the retinal vascular endothelial cells. One of the adhesion molecules of the endothelial cells, intercellular adhesion molecule-1 (ICAM-1), was up-regulated by the high glucose and insulin. The leukocyte adhesion was inhibited by anti-ICAM-1 antibody and the inhibitor of protein kinase C. These results suggested that both glucose and insulin increased the expression of ICAM-1 of the retinal vascular endothelial cells through the activation of protein kinase C and that the increased expression of ICAM-1 is the cause for the adhesion of netrophils.The effect of triamcinolone acetonide on the leukocyte adhesion after scatter retinal photocoagulation was studied in rats in vivo. Acridine orange fluorography was used to study leukocyte dynamics in the retina after laser treatment. The rolling of leukocy … More tes along the retinal veins and increased leukocyte entrapment were observed not only in the photocoagulated area but also in the untreated retina. The OCT study showed the development of retinal edema in the untreated area. The temporal increase in the level of VEGF in the retina and choroid was detected. These results suggest that temporal increase in the VEGF in the retina and choroid by scatter photocoagulation enhances retinal leukostasis which leads to development of retinal edema. We also studied the effect of sub-Tenon's injection of triamcinolone acetonide on the enhanced leukocyte-endothelial interaction and retinal edema after scatter retinal photocoagulation in rats. The results suggested that triamcinolne effectively inhibited the rolling and entrapment of leukocytes in the retina and improved the retinal edema after scatter photocoagulation. This supports the use of triamcinolone acetonide for patients with macular edema to prevent the worsening of macular edema after panretinal laser photocoagulation. Less

体外研究了白细胞对人视网膜血管内皮细胞的粘附。培养基中的高葡萄糖浓度和胰岛素增加了中性粒细胞对视网膜血管内皮细胞的粘附。内皮细胞的粘附分子之一，细胞间粘附分子-1（ICAM-1），在高葡萄糖和胰岛素的作用下上调。抗ICAM-1抗体和蛋白激酶C抑制剂抑制白细胞粘附。这些结果表明，葡萄糖和胰岛素均通过激活蛋白激酶C增加视网膜血管内皮细胞ICAM-1的表达，ICAM-1表达的增加是中性粒细胞粘附的原因。曲安奈德对白细胞粘附的影响在大鼠体内研究了散射视网膜光凝后的白细胞粘附。使用吖啶橙荧光检查来研究激光治疗后视网膜中的白细胞动态。不仅在光凝区域而且在未治疗的视网膜中都观察到白细胞沿着视网膜静脉滚动和白细胞滞留增加。 OCT 研究显示未治疗区域出现视网膜水肿。检测到视网膜和脉络膜中 VEGF 水平暂时增加。这些结果表明，通过散射光凝使视网膜和脉络膜中 VEGF 暂时增加，从而增强了视网膜白细胞停滞，从而导致视网膜水肿的发生。我们还研究了Tenon下注射曲安奈德对大鼠散射视网膜光凝后增强的白细胞-内皮相互作用和视网膜水肿的影响。结果提示，曲安西林能有效抑制视网膜白细胞的滚动和截留，改善散射光凝后的视网膜水肿。这支持黄斑水肿患者使用曲安奈德，以防止全视网膜激光光凝术后黄斑水肿恶化。较少的